Force-Induced Bidirectional Stepping of Cytoplasmic Dynein

Arne Gennerich, Andrew P. Carter, Samara L. Reck-Peterson, Ronald D. Vale

Research output: Contribution to journalArticle

250 Citations (Scopus)

Abstract

Cytoplasmic dynein is a minus-end-directed microtubule motor whose mechanism of movement remains poorly understood. Here, we use optical tweezers to examine the force-dependent stepping behavior of yeast cytoplasmic dynein. We find that dynein primarily advances in 8 nm increments but takes other sized steps (4-24 nm) as well. An opposing force induces more frequent backward stepping by dynein, and the motor walks backward toward the microtubule plus end at loads above its stall force of 7 pN. Remarkably, in the absence of ATP, dynein steps processively along microtubules under an external load, with less force required for minus-end- than for plus-end-directed movement. This nucleotide-independent walking reveals that force alone can drive repetitive microtubule detachment-attachment cycles of dynein's motor domains. These results suggest a model for how dynein's two motor domains coordinate their activities during normal processive motility and provide new clues for understanding dynein-based motility in living cells.

Original languageEnglish (US)
Pages (from-to)952-965
Number of pages14
JournalCell
Volume131
Issue number5
DOIs
StatePublished - Nov 30 2007
Externally publishedYes

Fingerprint

Cytoplasmic Dyneins
Dyneins
Microtubules
Optical Tweezers
Optical tweezers
Yeast
Walking
Nucleotides
Adenosine Triphosphate
Yeasts
Cells

Keywords

  • CHEMBIO
  • PROTEINS

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Gennerich, A., Carter, A. P., Reck-Peterson, S. L., & Vale, R. D. (2007). Force-Induced Bidirectional Stepping of Cytoplasmic Dynein. Cell, 131(5), 952-965. https://doi.org/10.1016/j.cell.2007.10.016

Force-Induced Bidirectional Stepping of Cytoplasmic Dynein. / Gennerich, Arne; Carter, Andrew P.; Reck-Peterson, Samara L.; Vale, Ronald D.

In: Cell, Vol. 131, No. 5, 30.11.2007, p. 952-965.

Research output: Contribution to journalArticle

Gennerich, A, Carter, AP, Reck-Peterson, SL & Vale, RD 2007, 'Force-Induced Bidirectional Stepping of Cytoplasmic Dynein', Cell, vol. 131, no. 5, pp. 952-965. https://doi.org/10.1016/j.cell.2007.10.016
Gennerich, Arne ; Carter, Andrew P. ; Reck-Peterson, Samara L. ; Vale, Ronald D. / Force-Induced Bidirectional Stepping of Cytoplasmic Dynein. In: Cell. 2007 ; Vol. 131, No. 5. pp. 952-965.
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