Food deprivation limits insulin secretory capacity in postpubertal rats

Patricia Vuguin, Xiaohui Ma, Xioman Yang, Manju Surana, Bing Liu, Nir Barzilai

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Because prenatal and perinatal undernutrition are associated with type 2 diabetes later in life, we posed the question whether nutrient deprivation during puberty would also result in a decreased ability to secrete insulin. Chronically catheterized, unstressed Sprague Dawley rats, fed ad libitum, were studied before puberty (Pre, n = 14) and after puberty (Post, n = 8). Moderately caloric-restricted rats (fed 70% of the control diet, n = 9), were studied after puberty. Insulin secretion was assessed using a hyperglycemic clamp at a glucose concentration of 300 mg/dL, or with a primed continuous infusion of intralipid (plasma FFA levels ∼1.5 mM) at a plasma glucose concentration of 200 mg/dL. Stimulated insulin levels increased in Post rats by 3- to 4-fold compared with Pre rats (from 4.6 ± 0.4 ng/mL Pre to 12.8 ± 0.7 ng/mL Post, and from 4.5 ± 0.4 ng/mL Pre to 15.8 ± 0.7 ng/mL Post, respectively, p < 0.001, at a glucose concentration of 300 mg/dL, and 200 mg/dL with intralipid). Caloric restriction prevented any rise in insulin secretion (3.8 ± 0.5 and 4.6 ± 0.5 ng/mL in the caloric-restricted rats at glucose concentrations of 300 mg/dL and 200 mg/dL with intralipid, respectively). A semiquantitative reverse-transcriptase PCR procedure was used to assess basal and stimulated insulin mRNA levels. Caloric restriction did not compensate by enhancing insulin mRNA levels in response to glucose stimulation. Moderate food deprivation during puberty reduced the capacity of the pancreas to secrete insulin in response to different nutrient stimuli. We hypothesize that puberty has an important role in β-cell maturation and any major nutrient modification may have deleterious consequences later in life.

Original languageEnglish (US)
Pages (from-to)468-473
Number of pages6
JournalPediatric Research
Volume49
Issue number4 I
StatePublished - 2001

Fingerprint

Food Deprivation
Puberty
Insulin
Glucose
Caloric Restriction
Food
Messenger RNA
Aptitude
Reverse Transcriptase Polymerase Chain Reaction
Malnutrition
Type 2 Diabetes Mellitus
Sprague Dawley Rats
Pancreas
Diet

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Vuguin, P., Ma, X., Yang, X., Surana, M., Liu, B., & Barzilai, N. (2001). Food deprivation limits insulin secretory capacity in postpubertal rats. Pediatric Research, 49(4 I), 468-473.

Food deprivation limits insulin secretory capacity in postpubertal rats. / Vuguin, Patricia; Ma, Xiaohui; Yang, Xioman; Surana, Manju; Liu, Bing; Barzilai, Nir.

In: Pediatric Research, Vol. 49, No. 4 I, 2001, p. 468-473.

Research output: Contribution to journalArticle

Vuguin, P, Ma, X, Yang, X, Surana, M, Liu, B & Barzilai, N 2001, 'Food deprivation limits insulin secretory capacity in postpubertal rats', Pediatric Research, vol. 49, no. 4 I, pp. 468-473.
Vuguin, Patricia ; Ma, Xiaohui ; Yang, Xioman ; Surana, Manju ; Liu, Bing ; Barzilai, Nir. / Food deprivation limits insulin secretory capacity in postpubertal rats. In: Pediatric Research. 2001 ; Vol. 49, No. 4 I. pp. 468-473.
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