TY - JOUR
T1 - Fluorescent proteins in cellular organelles
T2 - Serious pitfalls and some solutions
AU - Costantini, Lindsey M.
AU - Snapp, Erik Lee
PY - 2013/11/1
Y1 - 2013/11/1
N2 - Fluorescent proteins (FPs) have been powerful tools for cell biologists for over 15 years. The large variety of FPs available rarely comes with an instruction manual or a warning label. The potential pitfalls of the use of FPs in cellular organelles represent a significant concern for investigators. FPs generally did not evolve in the often distinctive physicochemical environments of subcellular organelles. In organelles, FPs can misfold, go dark, and even distort organelle morphology. In this minireview, we describe the issues associated with FPs in organelles and provide solutions to enable investigators to better exploit FP technology in cells.
AB - Fluorescent proteins (FPs) have been powerful tools for cell biologists for over 15 years. The large variety of FPs available rarely comes with an instruction manual or a warning label. The potential pitfalls of the use of FPs in cellular organelles represent a significant concern for investigators. FPs generally did not evolve in the often distinctive physicochemical environments of subcellular organelles. In organelles, FPs can misfold, go dark, and even distort organelle morphology. In this minireview, we describe the issues associated with FPs in organelles and provide solutions to enable investigators to better exploit FP technology in cells.
UR - http://www.scopus.com/inward/record.url?scp=84886077330&partnerID=8YFLogxK
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U2 - 10.1089/dna.2013.2172
DO - 10.1089/dna.2013.2172
M3 - Review article
C2 - 23971632
AN - SCOPUS:84886077330
SN - 1044-5498
VL - 32
SP - 622
EP - 627
JO - DNA and Cell Biology
JF - DNA and Cell Biology
IS - 11
ER -