Fidelity of translation in the presence of mammalian mitochondrial initiation factor 3

Shreya Ahana Ayyub, S. L. Aswathy, Divya Dobriyal, Srinivas Aluri, Linda L. Spremulli, Umesh Varshney

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Initiation factor 3 (IF3) is a conserved translation factor. Mutations in mitochondrial IF3 (IF3mt) have been implicated in disease pathology. Escherichia coli infCΔ55, compromised for IF3 activity, has provided an excellent heterologous system for IF3mt structure-function analysis. IF3mt allowed promiscuous initiation from AUA, AUU and ACG codons but avoided initiation with initiator tRNAs lacking the conserved 3GC pairs in their anticodon stems. Expression of IF3mt N-terminal domain, or IF3mt devoid of its typical N-, and C-terminal extensions improved fidelity of initiation in E. coli. The observations suggest that the IF3mt terminal extensions relax the fidelity of translational initiation in mitochondria.

Original languageEnglish (US)
Pages (from-to)1-8
Number of pages8
JournalMitochondrion
Volume39
DOIs
StatePublished - Mar 2018
Externally publishedYes

Keywords

  • Fidelity
  • Initiation factor 3
  • Mitochondria
  • Protein synthesis
  • Ribosome

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Cell Biology

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