Femtomolar inhibitors bind to 5′-methylthioadenosine nucleosidases with favorable enthalpy and entropy

Keisha Thomas, Antti M. Haapalainen, Emmanuel S. Burgos, Gary B. Evans, Peter C. Tyler, Shivali Gulab, Rong Guan, Vern L. Schramm

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

5′-Methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN) catalyzes the hydrolytic cleavage of adenine from methylthioadenosine (MTA). Inhibitor design and synthesis informed by transition state analysis have developed femtomolar inhibitors for MTANs, among the most powerful known noncovalent enzyme inhibitors. Thermodynamic analyses of the inhibitor binding reveals a combination of highly favorable contributions from enthalpic (-24.7 to -4.0 kcal mol-1) and entropic (-10.0 to 6.4 kcal mol-1) interactions. Inhibitor binding to similar MTANs from different bacterial species gave distinct energetic contributions from similar catalytic sites. Thus, binding of four transition state analogues to EcMTAN and SeMTAN is driven primarily by enthalpy, while binding to VcMTAN is driven primarily by entropy. Human MTA phosphorylase (hMTAP) has a transition state structure closely related to that of the bacterial MTANs, and it binds tightly to some of the same transition state analogues. However, the thermodynamic signature of binding of an inhibitor to hMTAP differs completely from that with MTANs. We conclude that factors other than first-sphere catalytic residue contacts contribute to binding of inhibitors because the thermodynamic signature differs between bacterial species of the same enzyme.

Original languageEnglish (US)
Pages (from-to)7541-7550
Number of pages10
JournalBiochemistry
Volume51
Issue number38
DOIs
StatePublished - Sep 25 2012

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Entropy
Thermodynamics
Enthalpy
adenosylhomocysteine nucleosidase
Pemetrexed
Phosphorylases
Enzyme Inhibitors
Adenine
Catalytic Domain
Enzymes
5'-methylthioadenosine phosphorylase
5'-methylthioadenosine

ASJC Scopus subject areas

  • Biochemistry

Cite this

Femtomolar inhibitors bind to 5′-methylthioadenosine nucleosidases with favorable enthalpy and entropy. / Thomas, Keisha; Haapalainen, Antti M.; Burgos, Emmanuel S.; Evans, Gary B.; Tyler, Peter C.; Gulab, Shivali; Guan, Rong; Schramm, Vern L.

In: Biochemistry, Vol. 51, No. 38, 25.09.2012, p. 7541-7550.

Research output: Contribution to journalArticle

Thomas, Keisha ; Haapalainen, Antti M. ; Burgos, Emmanuel S. ; Evans, Gary B. ; Tyler, Peter C. ; Gulab, Shivali ; Guan, Rong ; Schramm, Vern L. / Femtomolar inhibitors bind to 5′-methylthioadenosine nucleosidases with favorable enthalpy and entropy. In: Biochemistry. 2012 ; Vol. 51, No. 38. pp. 7541-7550.
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