Female genital tract secretions inhibit herpes simplex virus infection: Correlation with soluble mucosal immune mediators and impact of hormonal contraception

Gail F. Shust, Sylvia Cho, Mimi Kim, Rebecca P. Madan, Esmeralda M. Guzman, Margaret Pollack, Julia Epstein, Hillel W. Cohen, Marla J. Keller, Betsy Herold

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Problem: Female genital tract secretions inhibit herpes simplex virus (HSV) infection, however, the intra- and inter-subject variability, contribution of specific mediators, and impact of reproductive hormones have not been defined. Method of study: Cervicovaginal lavage (CVL) (n = 89) obtained from nine cyclers and seven women on hormonal contraception (HC), who completed between three and eight weekly visits, were examined for anti-herpes simplex virus activity and concentrations of mediators. Results: The CVL inhibited HSV infection by a mean value of approximately 57% during the follicular or luteal phase, but only by 36% in hormonal contraceptive users. Human neutrophil peptides 1-3 (HNP1-3) (P = 0.03), IL-8 (P = 0.003), lactoferrin (P = 0.005), lysozyme (P = 0.003), IgA (P = 0.002), and IgG (P = 0.02) correlated with antiviral activity. Intra-subject and inter-subject variability was observed, suggesting that factors other than hormones contribute to innate defense. Conclusion: Endogenous antimicrobial activity may provide a biomarker of healthy mucosal immunity and may be reduced in the setting of HC. However, larger prospective studies are needed.

Original languageEnglish (US)
Pages (from-to)110-119
Number of pages10
JournalAmerican Journal of Reproductive Immunology
Volume63
Issue number2
DOIs
StatePublished - Feb 2010

Fingerprint

Virus Diseases
Simplexvirus
Contraception
Therapeutic Irrigation
Hormones
Mucosal Immunity
Lactoferrin
Follicular Phase
Luteal Phase
Muramidase
Contraceptive Agents
Interleukin-8
Immunoglobulin A
Antiviral Agents
Immunoglobulin G
Biomarkers
Prospective Studies

Keywords

  • Genital herpes
  • Microbicides
  • Mucosal immunity
  • Reproductive hormones

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Obstetrics and Gynecology
  • Reproductive Medicine

Cite this

Female genital tract secretions inhibit herpes simplex virus infection : Correlation with soluble mucosal immune mediators and impact of hormonal contraception. / Shust, Gail F.; Cho, Sylvia; Kim, Mimi; Madan, Rebecca P.; Guzman, Esmeralda M.; Pollack, Margaret; Epstein, Julia; Cohen, Hillel W.; Keller, Marla J.; Herold, Betsy.

In: American Journal of Reproductive Immunology, Vol. 63, No. 2, 02.2010, p. 110-119.

Research output: Contribution to journalArticle

Shust, Gail F. ; Cho, Sylvia ; Kim, Mimi ; Madan, Rebecca P. ; Guzman, Esmeralda M. ; Pollack, Margaret ; Epstein, Julia ; Cohen, Hillel W. ; Keller, Marla J. ; Herold, Betsy. / Female genital tract secretions inhibit herpes simplex virus infection : Correlation with soluble mucosal immune mediators and impact of hormonal contraception. In: American Journal of Reproductive Immunology. 2010 ; Vol. 63, No. 2. pp. 110-119.
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