Factors influencing plant regeneration from protoplasts isolated from long-term cell suspension culture of recalcitrant indica rice cultivar IR36

K. Tang, Q. Hu, E. Zhao, A. Wu

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Factors influencing successful establishment of embryogenic cell-suspension cultures and plant regeneration from long-term cell suspension-derived protoplasts of the recalcitrant Indica rice cultivar IR36 were studied. The factors included cell and protoplast culture medium, protoplast culture procedure, the source of nurse cells, and the regeneration procedure. Embryogenic cell suspension cultures could only be established from mature seed-derived callus of IR36 in AA-based medium (Muller and Grafe, 1978). Protoplast-derived colonies could be obtained only using the filter-membrane nurse-culture procedure when Lolium multiflorum suspension cells served as nurse, rather than wild rice (Oryza ridleyi) and Japonica rice (Oryza sativa cv. Taipei 309) cells. The utilization of a two-step regeneration procedure led to regeneration of fertile plants from protoplasts isolated from 2-yr-old cell suspensions of IR36, one of the most important but recalcitrant rice cultivars.

Original languageEnglish (US)
Pages (from-to)255-259
Number of pages5
JournalIn Vitro Cellular and Developmental Biology - Plant
Volume36
Issue number4
StatePublished - 2000
Externally publishedYes

Fingerprint

Protoplasts
cell suspension culture
protoplasts
Regeneration
Suspensions
Cell Culture Techniques
rice
nurses
cultivars
Nurses
wild rice
Oryza
Lolium multiflorum
cells
Lolium
Bony Callus
Oryza sativa
callus
culture media
Culture Media

Keywords

  • Culture medium
  • IR36
  • Membrane-filter nurse culture
  • Two-step regeneration

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

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AU - Tang, K.

AU - Hu, Q.

AU - Zhao, E.

AU - Wu, A.

PY - 2000

Y1 - 2000

N2 - Factors influencing successful establishment of embryogenic cell-suspension cultures and plant regeneration from long-term cell suspension-derived protoplasts of the recalcitrant Indica rice cultivar IR36 were studied. The factors included cell and protoplast culture medium, protoplast culture procedure, the source of nurse cells, and the regeneration procedure. Embryogenic cell suspension cultures could only be established from mature seed-derived callus of IR36 in AA-based medium (Muller and Grafe, 1978). Protoplast-derived colonies could be obtained only using the filter-membrane nurse-culture procedure when Lolium multiflorum suspension cells served as nurse, rather than wild rice (Oryza ridleyi) and Japonica rice (Oryza sativa cv. Taipei 309) cells. The utilization of a two-step regeneration procedure led to regeneration of fertile plants from protoplasts isolated from 2-yr-old cell suspensions of IR36, one of the most important but recalcitrant rice cultivars.

AB - Factors influencing successful establishment of embryogenic cell-suspension cultures and plant regeneration from long-term cell suspension-derived protoplasts of the recalcitrant Indica rice cultivar IR36 were studied. The factors included cell and protoplast culture medium, protoplast culture procedure, the source of nurse cells, and the regeneration procedure. Embryogenic cell suspension cultures could only be established from mature seed-derived callus of IR36 in AA-based medium (Muller and Grafe, 1978). Protoplast-derived colonies could be obtained only using the filter-membrane nurse-culture procedure when Lolium multiflorum suspension cells served as nurse, rather than wild rice (Oryza ridleyi) and Japonica rice (Oryza sativa cv. Taipei 309) cells. The utilization of a two-step regeneration procedure led to regeneration of fertile plants from protoplasts isolated from 2-yr-old cell suspensions of IR36, one of the most important but recalcitrant rice cultivars.

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