Eye development

Nicholas E. Baker, Ke Li, Manon Quiquand, Robert Ruggiero, Lan Hsin Wang

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

The eye has been one of the most intensively studied organs in Drosophila. The wealth of knowledge about its development, as well as the reagents that have been developed, and the fact that the eye is dispensable for survival, also make the eye suitable for genetic interaction studies and genetic screens. This article provides a brief overview of the methods developed to image and probe eye development at multiple developmental stages, including live imaging, immunostaining of fixed tissues, in situ hybridizations, and scanning electron microscopy and color photography of adult eyes. Also summarized are genetic approaches that can be performed in the eye, including mosaic analysis and conditional mutation, gene misexpression and knockdown, and forward genetic and modifier screens.

Original languageEnglish (US)
Pages (from-to)252-259
Number of pages8
JournalMethods
Volume68
Issue number1
DOIs
StatePublished - Jun 15 2014

Keywords

  • Drosophila eye
  • Genetic screen
  • Immunocytochemistry
  • In situ hybridization
  • Live imaging
  • Scanning electron microscopy

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

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    Baker, N. E., Li, K., Quiquand, M., Ruggiero, R., & Wang, L. H. (2014). Eye development. Methods, 68(1), 252-259. https://doi.org/10.1016/j.ymeth.2014.04.007