Expression of Unmodified Hepatitis C Virus Envelope Glycoprotein-Coding Sequences Leads to Cryptic Intron Excision and Cell Surface Expression of E1/E2 Heterodimers Comprising Full-Length and Partially Deleted E1

Julie Dumonceaux, Emmanuel G. Cormier, Francis Kajumo, Gerald P. Donovan, Jayanta Roy-Chowdhury, Ira J. Fox, Jason P. Gardner, Tatjana Dragic

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Hepatitis C virus (HCV) is a positive-strand RNA virus that replicates exclusively in the cytoplasm of infected cells. The viral envelope glycoproteins, E1 and E2, appear to be retained in the endoplasmic reticulum, where viral budding is thought to occur. Surprisingly, we found that the expression system used to generate HCV envelope glycoproteins influences their subcellular localization and processing. These findings have important implications for optimizing novel HCV fusion and entry assays as well as for budding and virus particle formation.

Original languageEnglish (US)
Pages (from-to)13418-13424
Number of pages7
JournalJournal of virology
Volume77
Issue number24
DOIs
StatePublished - Dec 2003

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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