Expression of the crucifer-infecting TMV-Cg movement protein in tobacco plants complements in trans a TMV-U1 trafficking-deficient mutant

Felipe Diaz-Griffero, Carmen Espinoza Cancino, Consuelo Medina Arévalo, Patricio Arce-Johnson

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Tobamovirus movement proteins play a determinant role in the establishment of infections in plants, allowing the local movement of viral RNA genome through plasmodesmatas. We expressed the movement protein (MP) of the crucifer- and garlic-infecting Tobacco Mosaic Virus strain Cg (TMV-Cg) in both resistant Xanthi NN and sensitive Xanthi nn Nicotiana tabacum plants. MP-Cg function was assayed by inoculating transgenic plants with a trafficking-deficient mutant of TMV strain U1. Following infection, local necrotic lesions were developed in resistant transgenic plants, and a systemic infection was produced in sensitive tobaccos. Thus, movement function of the mutant virus was complemented in trans by MP-Cg expressed in transgenic plants, causing the same symptoms as wild-type strain. We demonstrated that the function of MP-U1 could be replaced efficiently by MP-Cg, even though these proteins share only 36% of identity. Similar hydrophobic patterns of MP-Cg and MP-U1 suggests structure and function conservations of both proteins. This work is an example of how two tobamoviruses differing in their host range help to understand viral movement mechanism during the infection.

Original languageEnglish (US)
Pages (from-to)269-279
Number of pages11
JournalBiological Research
Volume39
Issue number2
StatePublished - 2006
Externally publishedYes

Fingerprint

Tobacco Mosaic Virus
Tobacco mosaic virus
Tobacco
Viruses
complement
tobacco
mutants
Proteins
proteins
Genetically Modified Plants
Tobamovirus
transgenic plants
Infection
infection
lesions (plant)
Garlic
Viral Genome
Host Specificity
Viral RNA
garlic

Keywords

  • Crucifer-infecting tobamovirus
  • Movement protein
  • Viral complementation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Expression of the crucifer-infecting TMV-Cg movement protein in tobacco plants complements in trans a TMV-U1 trafficking-deficient mutant. / Diaz-Griffero, Felipe; Espinoza Cancino, Carmen; Medina Arévalo, Consuelo; Arce-Johnson, Patricio.

In: Biological Research, Vol. 39, No. 2, 2006, p. 269-279.

Research output: Contribution to journalArticle

Diaz-Griffero, Felipe ; Espinoza Cancino, Carmen ; Medina Arévalo, Consuelo ; Arce-Johnson, Patricio. / Expression of the crucifer-infecting TMV-Cg movement protein in tobacco plants complements in trans a TMV-U1 trafficking-deficient mutant. In: Biological Research. 2006 ; Vol. 39, No. 2. pp. 269-279.
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