Abstract
Tobamovirus movement proteins play a determinant role in the establishment of infections in plants, allowing the local movement of viral RNA genome through plasmodesmatas. We expressed the movement protein (MP) of the crucifer- and garlic-infecting Tobacco Mosaic Virus strain Cg (TMV-Cg) in both resistant Xanthi NN and sensitive Xanthi nn Nicotiana tabacum plants. MP-Cg function was assayed by inoculating transgenic plants with a trafficking-deficient mutant of TMV strain U1. Following infection, local necrotic lesions were developed in resistant transgenic plants, and a systemic infection was produced in sensitive tobaccos. Thus, movement function of the mutant virus was complemented in trans by MP-Cg expressed in transgenic plants, causing the same symptoms as wild-type strain. We demonstrated that the function of MP-U1 could be replaced efficiently by MP-Cg, even though these proteins share only 36% of identity. Similar hydrophobic patterns of MP-Cg and MP-U1 suggests structure and function conservations of both proteins. This work is an example of how two tobamoviruses differing in their host range help to understand viral movement mechanism during the infection.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 269-279 |
| Number of pages | 11 |
| Journal | Biological Research |
| Volume | 39 |
| Issue number | 2 |
| State | Published - 2006 |
| Externally published | Yes |
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Keywords
- Crucifer-infecting tobamovirus
- Movement protein
- Viral complementation
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
Cite this
Expression of the crucifer-infecting TMV-Cg movement protein in tobacco plants complements in trans a TMV-U1 trafficking-deficient mutant. / Diaz-Griffero, Felipe; Espinoza Cancino, Carmen; Medina Arévalo, Consuelo; Arce-Johnson, Patricio.
In: Biological Research, Vol. 39, No. 2, 2006, p. 269-279.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of the crucifer-infecting TMV-Cg movement protein in tobacco plants complements in trans a TMV-U1 trafficking-deficient mutant
AU - Diaz-Griffero, Felipe
AU - Espinoza Cancino, Carmen
AU - Medina Arévalo, Consuelo
AU - Arce-Johnson, Patricio
PY - 2006
Y1 - 2006
N2 - Tobamovirus movement proteins play a determinant role in the establishment of infections in plants, allowing the local movement of viral RNA genome through plasmodesmatas. We expressed the movement protein (MP) of the crucifer- and garlic-infecting Tobacco Mosaic Virus strain Cg (TMV-Cg) in both resistant Xanthi NN and sensitive Xanthi nn Nicotiana tabacum plants. MP-Cg function was assayed by inoculating transgenic plants with a trafficking-deficient mutant of TMV strain U1. Following infection, local necrotic lesions were developed in resistant transgenic plants, and a systemic infection was produced in sensitive tobaccos. Thus, movement function of the mutant virus was complemented in trans by MP-Cg expressed in transgenic plants, causing the same symptoms as wild-type strain. We demonstrated that the function of MP-U1 could be replaced efficiently by MP-Cg, even though these proteins share only 36% of identity. Similar hydrophobic patterns of MP-Cg and MP-U1 suggests structure and function conservations of both proteins. This work is an example of how two tobamoviruses differing in their host range help to understand viral movement mechanism during the infection.
AB - Tobamovirus movement proteins play a determinant role in the establishment of infections in plants, allowing the local movement of viral RNA genome through plasmodesmatas. We expressed the movement protein (MP) of the crucifer- and garlic-infecting Tobacco Mosaic Virus strain Cg (TMV-Cg) in both resistant Xanthi NN and sensitive Xanthi nn Nicotiana tabacum plants. MP-Cg function was assayed by inoculating transgenic plants with a trafficking-deficient mutant of TMV strain U1. Following infection, local necrotic lesions were developed in resistant transgenic plants, and a systemic infection was produced in sensitive tobaccos. Thus, movement function of the mutant virus was complemented in trans by MP-Cg expressed in transgenic plants, causing the same symptoms as wild-type strain. We demonstrated that the function of MP-U1 could be replaced efficiently by MP-Cg, even though these proteins share only 36% of identity. Similar hydrophobic patterns of MP-Cg and MP-U1 suggests structure and function conservations of both proteins. This work is an example of how two tobamoviruses differing in their host range help to understand viral movement mechanism during the infection.
KW - Crucifer-infecting tobamovirus
KW - Movement protein
KW - Viral complementation
UR - http://www.scopus.com/inward/record.url?scp=33746650416&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33746650416&partnerID=8YFLogxK
M3 - Article
C2 - 16874402
AN - SCOPUS:33746650416
VL - 39
SP - 269
EP - 279
JO - Biological Research
JF - Biological Research
SN - 0716-9760
IS - 2
ER -