Abstract
A cloned human cardiac actin gene, introduced into mouse Ltk- cells, is expressed in several thymidine kinase (tk)-positive cotransfectants. The clones not only produce authentic polyadenylated human cardiac actin mRNA but also synthesize human cardiac actin protein. The cardiac actin protein, normally found only in myofibrils, is stably accumulated at a high level, about one-third that of the endogenous mouse β-actin. Furthermore, this sarcomeric protein partitions between the Triton X-100 insoluble and soluble phases to the same extent as the endogenous β-actin. This suggests that a sarcomeric actin can participate in the formation of Triton X-100-insoluble cytoskeletal structures.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 709-715 |
| Number of pages | 7 |
| Journal | Cell |
| Volume | 36 |
| Issue number | 3 |
| DOIs | |
| State | Published - 1984 |
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ASJC Scopus subject areas
- Cell Biology
- Molecular Biology
- Medicine(all)
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Expression of human cardiac actin in mouse L cells : A sarcomeric actin associates with a nonmuscle cytoskeleton. / Gunning, Peter; Ponte, Phyllis; Kedes, Larry; Hickey, Robert J.; Skoultchi, Arthur I.
In: Cell, Vol. 36, No. 3, 1984, p. 709-715.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of human cardiac actin in mouse L cells
T2 - A sarcomeric actin associates with a nonmuscle cytoskeleton
AU - Gunning, Peter
AU - Ponte, Phyllis
AU - Kedes, Larry
AU - Hickey, Robert J.
AU - Skoultchi, Arthur I.
PY - 1984
Y1 - 1984
N2 - A cloned human cardiac actin gene, introduced into mouse Ltk- cells, is expressed in several thymidine kinase (tk)-positive cotransfectants. The clones not only produce authentic polyadenylated human cardiac actin mRNA but also synthesize human cardiac actin protein. The cardiac actin protein, normally found only in myofibrils, is stably accumulated at a high level, about one-third that of the endogenous mouse β-actin. Furthermore, this sarcomeric protein partitions between the Triton X-100 insoluble and soluble phases to the same extent as the endogenous β-actin. This suggests that a sarcomeric actin can participate in the formation of Triton X-100-insoluble cytoskeletal structures.
AB - A cloned human cardiac actin gene, introduced into mouse Ltk- cells, is expressed in several thymidine kinase (tk)-positive cotransfectants. The clones not only produce authentic polyadenylated human cardiac actin mRNA but also synthesize human cardiac actin protein. The cardiac actin protein, normally found only in myofibrils, is stably accumulated at a high level, about one-third that of the endogenous mouse β-actin. Furthermore, this sarcomeric protein partitions between the Triton X-100 insoluble and soluble phases to the same extent as the endogenous β-actin. This suggests that a sarcomeric actin can participate in the formation of Triton X-100-insoluble cytoskeletal structures.
UR - http://www.scopus.com/inward/record.url?scp=0021282350&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021282350&partnerID=8YFLogxK
U2 - 10.1016/0092-8674(84)90351-9
DO - 10.1016/0092-8674(84)90351-9
M3 - Article
VL - 36
SP - 709
EP - 715
JO - Cell
JF - Cell
SN - 0092-8674
IS - 3
ER -