Expression of cloned sequences in biopsies of human colonic tissue and in colonic carcinoma cells induced to differentiate in vitro

Leonard H. Augenlicht, M. Z. Wahrman, H. Halsey, L. Anderson, J. Taylor, M. Lipkin

Research output: Contribution to journalArticle

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Abstract

A computer-based scanning and image-processing system has been developed to quantitate the relative level of expression of each of 4000 cloned complementary DNA sequences in small biopsies routinely removed from the mucosa of normal and neoplastic human large intestine. Individuals have been studied from well-defined population groups in which colonic epithelial cells have progressed to increasingly advanced stages of neoplastic transformation. Comparison of normal colonic mucosa to colonic carcinomas demonstrated alterations in expression of approximately 7% of the cloned sequences; fewer changes were found between benign colonic adenomas and either normal colonic mucosa or carcinomas. A subset of the sequences which change in expression during progression from normal mucosa, to adenoma, to carcinoma showed complementary changes when colon carcinoma cells were induced to differentiate in vitro with sodium butyrate; quantitative correlations between in vivo and in vitro results were highly significant. Comparison of normal colonic mucosa with mucosa from patients with the autosomal dominant disease familial polyposis revealed more extensive alterations in gene expression involving approximately 25% of the clones screened. Flat colonic mucosa in familial polyposis is therefore markedly aberrant and may be highly dedifferentiated, suggesting several possible mechanisms for the very high incidence of cancer that develops in this epithelium.

Original languageEnglish (US)
Pages (from-to)6017-6021
Number of pages5
JournalCancer Research
Volume47
Issue number22
StatePublished - 1987

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Mucous Membrane
Carcinoma
Biopsy
Adenoma
Butyric Acid
Large Intestine
In Vitro Techniques
Population Groups
Colon
Epithelium
Complementary DNA
Clone Cells
Epithelial Cells
Gene Expression
Incidence
Neoplasms

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Expression of cloned sequences in biopsies of human colonic tissue and in colonic carcinoma cells induced to differentiate in vitro. / Augenlicht, Leonard H.; Wahrman, M. Z.; Halsey, H.; Anderson, L.; Taylor, J.; Lipkin, M.

In: Cancer Research, Vol. 47, No. 22, 1987, p. 6017-6021.

Research output: Contribution to journalArticle

Augenlicht, Leonard H. ; Wahrman, M. Z. ; Halsey, H. ; Anderson, L. ; Taylor, J. ; Lipkin, M. / Expression of cloned sequences in biopsies of human colonic tissue and in colonic carcinoma cells induced to differentiate in vitro. In: Cancer Research. 1987 ; Vol. 47, No. 22. pp. 6017-6021.
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