Expression and signal transduction of the glucagon receptor in βTC3 cells

Jing Li, Jorge N. Larocca, Alicia G. Rodriguez-Gabin, Maureen J. Charron

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

The expression and signal transduction of the glucagon receptor (GR) have been studied in βTC3 cells. Northern blot and RT-PCR analysis indicated the expression of the GR gene in βTC3 cells. One-5 nM glucagon stimulated a 2.5-fold increase in the IP(s) production. At glucagon concentrations higher than 5 nM, the production of IP(s) was blunted but not abolished. The accumulation of intracellular cAMP was observed following the stimulation with 5 nM of glucagon. A maximal 4.5-fold increase in cAMP was observed using 250 nM glucagon and higher. Comparative studies using a glucagon antagonist, des-His1[Glu]9 glucagon, showed no effect on intracellular cAMP and IPs in βTC3 cells. Our data shows that the GR gene is expressed in βTC3 cells. The GR in βTC3 cells transmits its intracellular signal by causing the accumulation of both IP(s) and cAMP.

Original languageEnglish (US)
Pages (from-to)229-236
Number of pages8
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1356
Issue number2
DOIs
StatePublished - Apr 24 1997

Keywords

  • Cyclic AMP
  • Glucagon receptor
  • Inositol phosphate
  • βTC3 cells

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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