Expression and activities of matrix metalloproteinases under oscillatory shear in IL-1-stimulated synovial cells

Hui (Herb) Sun, Razi Nalim, Hiroki Yokota

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Matrix metalloproteinases (MMPs) are known to play a critical role in tissue disintegration, and an elevated level of MMPs is observed in synovium and synovial fluid of joints with rheumatoid arthritis. During joint movement, synovial tissue receives various mechanical stimuli, but effects of mechanical challenges on regulation of MMPs in rheumatic synovium are poorly understood. Focusing on cellular responses to oscillatory fluid shear in human synovial cells, we determined the expression of MMP-1 and MMP-13 by polymerase chain reaction and immunoblotting as well as proteolytic activities of total MMPs by a fibril degradation assay and zymography. The results revealed that ∼0.5 dyn/cm2 oscillatory shear at 1 Hz not only reduced an mRNA level and a protein level of MMP-1 and MMP-13, but it also decreased collagenase and gelatinase activities of total MMPs. Furthermore, the induction of the MMP expression and activities by interleukin-1 was suppressed by the oscillatory shear. Interestingly, the oscillatory shear upregulated the mRNA expression of TIMP-1 and TIMP-2. Our results support a potential role of oscillatory shear in regulating expression and activities of MMPs in the presence and the absence of proinflammatory cytokine.

Original languageEnglish (US)
Pages (from-to)42-49
Number of pages8
JournalConnective Tissue Research
Volume44
Issue number1
StatePublished - 2003
Externally publishedYes

Fingerprint

Matrix Metalloproteinases
Interleukin-1
Matrix Metalloproteinase 13
Matrix Metalloproteinase 1
Synovial Membrane
Joints
Tissue
Tissue Inhibitor of Metalloproteinase-2
Gelatinases
Messenger RNA
Tissue Inhibitor of Metalloproteinase-1
Fluids
Disintegration
Polymerase chain reaction
Synovial Fluid
Collagenases
Immunoblotting
Assays
Rheumatoid Arthritis
Cytokines

Keywords

  • Interleukin 1
  • MMP
  • Rheumatoid arthritis
  • Shear Stress
  • Synovium

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Nephrology
  • Cell Biology
  • Orthopedics and Sports Medicine

Cite this

Expression and activities of matrix metalloproteinases under oscillatory shear in IL-1-stimulated synovial cells. / Sun, Hui (Herb); Nalim, Razi; Yokota, Hiroki.

In: Connective Tissue Research, Vol. 44, No. 1, 2003, p. 42-49.

Research output: Contribution to journalArticle

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