Exponential growth by cross-catalytic cleavage of deoxyribozymogens

Matthew Levy, Andrew D. Ellington

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

We have designed an autocatalytic cycle based on the highly efficient 10-23 RNA-cleaving deoxyribozyme that is capable of exponential amplification of catalysis. In this system, complementary 10-23 variants were inactivated by circularization, creating deoxyribozymogens. Upon linearization, the enzymes can act on their complements, creating a cascade in which linearized species accumulate exponentially. Seeding the system with a pool of linear catalysts resulted not only in amplification of function but in sequence selection and represents an in vitro selection experiment conducted in the absence of any protein enzymes.

Original languageEnglish (US)
Pages (from-to)6416-6421
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume100
Issue number11
DOIs
StatePublished - May 27 2003
Externally publishedYes

Fingerprint

Catalytic DNA
Enzymes
Growth
Catalysis
RNA
Proteins
In Vitro Techniques

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Exponential growth by cross-catalytic cleavage of deoxyribozymogens. / Levy, Matthew; Ellington, Andrew D.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, No. 11, 27.05.2003, p. 6416-6421.

Research output: Contribution to journalArticle

@article{0654aedc07bb403eb988bb65dc8393ae,
title = "Exponential growth by cross-catalytic cleavage of deoxyribozymogens",
abstract = "We have designed an autocatalytic cycle based on the highly efficient 10-23 RNA-cleaving deoxyribozyme that is capable of exponential amplification of catalysis. In this system, complementary 10-23 variants were inactivated by circularization, creating deoxyribozymogens. Upon linearization, the enzymes can act on their complements, creating a cascade in which linearized species accumulate exponentially. Seeding the system with a pool of linear catalysts resulted not only in amplification of function but in sequence selection and represents an in vitro selection experiment conducted in the absence of any protein enzymes.",
author = "Matthew Levy and Ellington, {Andrew D.}",
year = "2003",
month = "5",
day = "27",
doi = "10.1073/pnas.1130145100",
language = "English (US)",
volume = "100",
pages = "6416--6421",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "11",

}

TY - JOUR

T1 - Exponential growth by cross-catalytic cleavage of deoxyribozymogens

AU - Levy, Matthew

AU - Ellington, Andrew D.

PY - 2003/5/27

Y1 - 2003/5/27

N2 - We have designed an autocatalytic cycle based on the highly efficient 10-23 RNA-cleaving deoxyribozyme that is capable of exponential amplification of catalysis. In this system, complementary 10-23 variants were inactivated by circularization, creating deoxyribozymogens. Upon linearization, the enzymes can act on their complements, creating a cascade in which linearized species accumulate exponentially. Seeding the system with a pool of linear catalysts resulted not only in amplification of function but in sequence selection and represents an in vitro selection experiment conducted in the absence of any protein enzymes.

AB - We have designed an autocatalytic cycle based on the highly efficient 10-23 RNA-cleaving deoxyribozyme that is capable of exponential amplification of catalysis. In this system, complementary 10-23 variants were inactivated by circularization, creating deoxyribozymogens. Upon linearization, the enzymes can act on their complements, creating a cascade in which linearized species accumulate exponentially. Seeding the system with a pool of linear catalysts resulted not only in amplification of function but in sequence selection and represents an in vitro selection experiment conducted in the absence of any protein enzymes.

UR - http://www.scopus.com/inward/record.url?scp=0038652093&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0038652093&partnerID=8YFLogxK

U2 - 10.1073/pnas.1130145100

DO - 10.1073/pnas.1130145100

M3 - Article

C2 - 12743371

AN - SCOPUS:0038652093

VL - 100

SP - 6416

EP - 6421

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 11

ER -