Exponential growth by cross-catalytic cleavage of deoxyribozymogens

Matthew Levy, Andrew D. Ellington

Research output: Contribution to journalArticle

61 Scopus citations

Abstract

We have designed an autocatalytic cycle based on the highly efficient 10-23 RNA-cleaving deoxyribozyme that is capable of exponential amplification of catalysis. In this system, complementary 10-23 variants were inactivated by circularization, creating deoxyribozymogens. Upon linearization, the enzymes can act on their complements, creating a cascade in which linearized species accumulate exponentially. Seeding the system with a pool of linear catalysts resulted not only in amplification of function but in sequence selection and represents an in vitro selection experiment conducted in the absence of any protein enzymes.

Original languageEnglish (US)
Pages (from-to)6416-6421
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume100
Issue number11
DOIs
Publication statusPublished - May 27 2003

    Fingerprint

ASJC Scopus subject areas

  • General

Cite this