Evidence for possible nonspecific reactions between N-ethylmaleimide and proteins

Curtis F. Brewer, John P. Riehm

Research output: Contribution to journalArticle

183 Citations (Scopus)

Abstract

The reaction between NEM and the two proteins lysozyme and ribonuclease A results, under certain experimental conditions, in the alkylation of ε{lunate}-amino and imidazole groups. The extent of reaction may be determined by amino acid analysis since the reaction products yield ninhydrin-positive zones.

Original languageEnglish (US)
Pages (from-to)248-255
Number of pages8
JournalAnalytical Biochemistry
Volume18
Issue number2
StatePublished - Feb 1967
Externally publishedYes

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Ninhydrin
Pancreatic Ribonuclease
Ethylmaleimide
Alkylation
Muramidase
Reaction products
Amino Acids
Proteins
imidazole

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Cite this

Evidence for possible nonspecific reactions between N-ethylmaleimide and proteins. / Brewer, Curtis F.; Riehm, John P.

In: Analytical Biochemistry, Vol. 18, No. 2, 02.1967, p. 248-255.

Research output: Contribution to journalArticle

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