Evidence for possible nonspecific reactions between N-ethylmaleimide and proteins

Curtis F. Brewer, John P. Riehm

Research output: Contribution to journalArticlepeer-review

194 Scopus citations

Abstract

The reaction between NEM and the two proteins lysozyme and ribonuclease A results, under certain experimental conditions, in the alkylation of ε{lunate}-amino and imidazole groups. The extent of reaction may be determined by amino acid analysis since the reaction products yield ninhydrin-positive zones.

Original languageEnglish (US)
Pages (from-to)248-255
Number of pages8
JournalAnalytical Biochemistry
Volume18
Issue number2
DOIs
StatePublished - Feb 1967
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Evidence for possible nonspecific reactions between N-ethylmaleimide and proteins'. Together they form a unique fingerprint.

Cite this