Evidence for a transposon in caenorhabditis elegans

Scott W. Emmons; Lewis Yesner; Ke san Ruan; Daniel Katzenberg

doi:10.1016/0092-8674(83)90496-8

Evidence for a transposon in caenorhabditis elegans

Scott W. Emmons, Lewis Yesner, Ke san Ruan, Daniel Katzenberg

Genetics

Research output: Contribution to journal › Article › peer-review

166 Scopus citations

Abstract

The C. elegans genome contains a 1.7 kb repeated DNA sequence (Tc1) that is present in different numbers in various strains. In strain Bristol and 10 other strains analyzed, there are 20 ± 5 copies of Tc1, and these are located at a nearly constant set of sites in the DNA. In Bergerac, however, there are 200 ± 50 interspersed copies of Tc1 that have arisen by insertion of Tc1 elements into new genomic sites. The interspersed copies of Tc1 have a conserved, nonpermuted structure. The structure of genomic Tc1 elements was analyzed by the cloning of a single Tc1 element from Bergerac and the comparison of its structure with homologous genomic sequences in Bristol and Bergerac. Tc1 elements at three sites analyzed in Bergerac undergo apparently precise excision from their points of insertion at high frequency.

Original language	English (US)
Pages (from-to)	55-65
Number of pages	11
Journal	Cell
Volume	32
Issue number	1
DOIs	https://doi.org/10.1016/0092-8674(83)90496-8
State	Published - Jan 1983

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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title = "Evidence for a transposon in caenorhabditis elegans",

abstract = "The C. elegans genome contains a 1.7 kb repeated DNA sequence (Tc1) that is present in different numbers in various strains. In strain Bristol and 10 other strains analyzed, there are 20 ± 5 copies of Tc1, and these are located at a nearly constant set of sites in the DNA. In Bergerac, however, there are 200 ± 50 interspersed copies of Tc1 that have arisen by insertion of Tc1 elements into new genomic sites. The interspersed copies of Tc1 have a conserved, nonpermuted structure. The structure of genomic Tc1 elements was analyzed by the cloning of a single Tc1 element from Bergerac and the comparison of its structure with homologous genomic sequences in Bristol and Bergerac. Tc1 elements at three sites analyzed in Bergerac undergo apparently precise excision from their points of insertion at high frequency.",

author = "Emmons, {Scott W.} and Lewis Yesner and Ruan, {Ke san} and Daniel Katzenberg",

note = "Funding Information: We thank Dr. D. Hirsh for sharing materials and results obtained in parallel experiments in his laboratory, and Drs. G. Childs, L. Lein-wand, L. Shapiro and P. Silverman for their comments on the manuscript. This work was supported by a United States Public Health Service grant to S. E. S. E. is the recipient of a Faculty Research Award from the American Cancer Society. D. K. is also supported by a United States Public Health Service grant. One nematode strain used in this work was provided by the Caenorhabditis Genetics Center, which is supported by a contract between the NIH and the Curators of the University of Missouri.",

year = "1983",

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doi = "10.1016/0092-8674(83)90496-8",

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AU - Yesner, Lewis

AU - Ruan, Ke san

AU - Katzenberg, Daniel

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PY - 1983/1

Y1 - 1983/1

N2 - The C. elegans genome contains a 1.7 kb repeated DNA sequence (Tc1) that is present in different numbers in various strains. In strain Bristol and 10 other strains analyzed, there are 20 ± 5 copies of Tc1, and these are located at a nearly constant set of sites in the DNA. In Bergerac, however, there are 200 ± 50 interspersed copies of Tc1 that have arisen by insertion of Tc1 elements into new genomic sites. The interspersed copies of Tc1 have a conserved, nonpermuted structure. The structure of genomic Tc1 elements was analyzed by the cloning of a single Tc1 element from Bergerac and the comparison of its structure with homologous genomic sequences in Bristol and Bergerac. Tc1 elements at three sites analyzed in Bergerac undergo apparently precise excision from their points of insertion at high frequency.

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Evidence for a transposon in caenorhabditis elegans

Abstract

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