Evaluation of the antibiotic properties of glutathione

David O. Schairer, Jason S. Chouake, Allison J. Kutnery, Joy Makdisi, Joshua D. Nosanchuk, Adam J. Friedman

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Skin and soft tissue infections (SSTIs) are growing in prevalence in both the outpatient and inpatient settings and are some of the most common diseases seen by dermatologists, who are often the first point of care for these patients. Microbial resistance to antibiotics continues to rise as more virulent strains evolve, and strains predominantly found in the hospital setting are now being seen in the community. Therefore, innovative approaches to combat this trend are needed. Glutathione (GSH) is a well-described and established antioxidant. It participates in detoxification of xenobiotics, regulation of cellular growth, modulation of immune response, and maintenance of the thiol status of proteins and cellular cysteine levels. GSH is also known to have a regulatory effect on immune cells and even inherent antibacterial properties have been reported. To this end, the value of GSH as an antibiotic was evaluated by growing methicillin resistant S. aureus, E. coli, K. pneumoniae and P. aeruginosa strains isolated from human skin and soft tissue infection in the presence of GSH. At a physiologic concentration of 10mM, GSH had no effect on bacterial growth. At concentrations above 50 mM, which created acidic conditions (pH < 4), bacterial growth was completely inhibited. When adjusted to physiologic pH, GSH exhibited a bacteriostatic effect in a concentration- dependent manner. Additionally, the cytotoxicity of GSH was evaluated in a murine cell line. GSH was relatively non-toxic to murine macrophages, even at the highest concentration tested (160 mM). These results suggest the potential utility of GSH for the prevention and/or as adjunctive treatment of infection, most significantly in disease states associated with GSH deficiency.

Original languageEnglish (US)
Pages (from-to)1272-1277
Number of pages6
JournalJournal of Drugs in Dermatology
Volume12
Issue number11
StatePublished - Nov 2013

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Glutathione
Soft Tissue Infections
Anti-Bacterial Agents
Growth
Point-of-Care Systems
Skin
Methicillin Resistance
Xenobiotics
Microbial Drug Resistance
Sulfhydryl Compounds
Cysteine
Inpatients
Pneumonia
Outpatients
Antioxidants
Macrophages
Maintenance
Escherichia coli
Cell Line
Infection

ASJC Scopus subject areas

  • Dermatology

Cite this

Schairer, D. O., Chouake, J. S., Kutnery, A. J., Makdisi, J., Nosanchuk, J. D., & Friedman, A. J. (2013). Evaluation of the antibiotic properties of glutathione. Journal of Drugs in Dermatology, 12(11), 1272-1277.

Evaluation of the antibiotic properties of glutathione. / Schairer, David O.; Chouake, Jason S.; Kutnery, Allison J.; Makdisi, Joy; Nosanchuk, Joshua D.; Friedman, Adam J.

In: Journal of Drugs in Dermatology, Vol. 12, No. 11, 11.2013, p. 1272-1277.

Research output: Contribution to journalArticle

Schairer, DO, Chouake, JS, Kutnery, AJ, Makdisi, J, Nosanchuk, JD & Friedman, AJ 2013, 'Evaluation of the antibiotic properties of glutathione', Journal of Drugs in Dermatology, vol. 12, no. 11, pp. 1272-1277.
Schairer DO, Chouake JS, Kutnery AJ, Makdisi J, Nosanchuk JD, Friedman AJ. Evaluation of the antibiotic properties of glutathione. Journal of Drugs in Dermatology. 2013 Nov;12(11):1272-1277.
Schairer, David O. ; Chouake, Jason S. ; Kutnery, Allison J. ; Makdisi, Joy ; Nosanchuk, Joshua D. ; Friedman, Adam J. / Evaluation of the antibiotic properties of glutathione. In: Journal of Drugs in Dermatology. 2013 ; Vol. 12, No. 11. pp. 1272-1277.
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