Evaluation of B-cell immunity in patients with pretransplant sensitization

Dimitri S. Monos, Michael B. Prystowsky, Arnold I. Levinson, Chester M. Zmijewski

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The influence of presensitization (blood transfusions) on B-cell immunity as reflected in the serum of two groups of candidates for cadaveric donor renal allografts was examined. The first group initially had a high level of panel-reactive antibody (PRA) greater than 72% but experienced a large decrease in PRA (>70%) 6-34 months prior to transplantation. In contrast, the second group maintained a high PRA (100%) for up to 28 months after sensitization and before transplantation. Three blood samples from each patient, representing a maximum time span of 34 months, were analyzed. Levels of IgG, IgM isohemagglutinins, and antitetanus antibody were used as indicators of B-cell function. There were no significant differences between the individual values of a single patient with regard to each parameter. However, Group II patients had elevated values of total IgG relative to Group I patients. Total serum IgG-subclass levels (IgG1, IgG2, IgG3, IgG4) were measured and the relationship between a specific IgG subclass and the PRA activity was determined. IgG1 values in Group II were higher than those found for Group I. The other IgG subclasses were all within normal levels and were not significantly different between Group I and Group II. When IgG-subclass typing of PRA was performed, IgG1 accounted for most of the activity in both groups and a fall in PRA-specific IgG1 was associated with the reduced PRA observed in Group I. The data indicate that humoral immunity, as reflected by total and specific immunoglobulin levels, is intact in general in the two groups of presensitized renal allograft candidates examined and that any loss of PRA activity reflects a reduction in a specific immune response.

Original languageEnglish (US)
Pages (from-to)200-206
Number of pages7
JournalJournal of Clinical Immunology
Volume8
Issue number3
DOIs
StatePublished - May 1988
Externally publishedYes

Fingerprint

Immunity
B-Lymphocytes
Immunoglobulin G
Antibodies
Allografts
Transplantation
Kidney
Hemagglutinins
Humoral Immunity
Serum
Individuality
Blood Transfusion
Immunoglobulin M
Immunoglobulins
Tissue Donors

Keywords

  • B-cell immunity
  • IgG subclasses
  • panel-reactive antibodies
  • Pretransplant sensitization

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Evaluation of B-cell immunity in patients with pretransplant sensitization. / Monos, Dimitri S.; Prystowsky, Michael B.; Levinson, Arnold I.; Zmijewski, Chester M.

In: Journal of Clinical Immunology, Vol. 8, No. 3, 05.1988, p. 200-206.

Research output: Contribution to journalArticle

Monos, Dimitri S. ; Prystowsky, Michael B. ; Levinson, Arnold I. ; Zmijewski, Chester M. / Evaluation of B-cell immunity in patients with pretransplant sensitization. In: Journal of Clinical Immunology. 1988 ; Vol. 8, No. 3. pp. 200-206.
@article{ce396e8621a74fddbcf41e8190be8adb,
title = "Evaluation of B-cell immunity in patients with pretransplant sensitization",
abstract = "The influence of presensitization (blood transfusions) on B-cell immunity as reflected in the serum of two groups of candidates for cadaveric donor renal allografts was examined. The first group initially had a high level of panel-reactive antibody (PRA) greater than 72{\%} but experienced a large decrease in PRA (>70{\%}) 6-34 months prior to transplantation. In contrast, the second group maintained a high PRA (100{\%}) for up to 28 months after sensitization and before transplantation. Three blood samples from each patient, representing a maximum time span of 34 months, were analyzed. Levels of IgG, IgM isohemagglutinins, and antitetanus antibody were used as indicators of B-cell function. There were no significant differences between the individual values of a single patient with regard to each parameter. However, Group II patients had elevated values of total IgG relative to Group I patients. Total serum IgG-subclass levels (IgG1, IgG2, IgG3, IgG4) were measured and the relationship between a specific IgG subclass and the PRA activity was determined. IgG1 values in Group II were higher than those found for Group I. The other IgG subclasses were all within normal levels and were not significantly different between Group I and Group II. When IgG-subclass typing of PRA was performed, IgG1 accounted for most of the activity in both groups and a fall in PRA-specific IgG1 was associated with the reduced PRA observed in Group I. The data indicate that humoral immunity, as reflected by total and specific immunoglobulin levels, is intact in general in the two groups of presensitized renal allograft candidates examined and that any loss of PRA activity reflects a reduction in a specific immune response.",
keywords = "B-cell immunity, IgG subclasses, panel-reactive antibodies, Pretransplant sensitization",
author = "Monos, {Dimitri S.} and Prystowsky, {Michael B.} and Levinson, {Arnold I.} and Zmijewski, {Chester M.}",
year = "1988",
month = "5",
doi = "10.1007/BF00917567",
language = "English (US)",
volume = "8",
pages = "200--206",
journal = "Journal of Clinical Immunology",
issn = "0271-9142",
publisher = "Springer New York",
number = "3",

}

TY - JOUR

T1 - Evaluation of B-cell immunity in patients with pretransplant sensitization

AU - Monos, Dimitri S.

AU - Prystowsky, Michael B.

AU - Levinson, Arnold I.

AU - Zmijewski, Chester M.

PY - 1988/5

Y1 - 1988/5

N2 - The influence of presensitization (blood transfusions) on B-cell immunity as reflected in the serum of two groups of candidates for cadaveric donor renal allografts was examined. The first group initially had a high level of panel-reactive antibody (PRA) greater than 72% but experienced a large decrease in PRA (>70%) 6-34 months prior to transplantation. In contrast, the second group maintained a high PRA (100%) for up to 28 months after sensitization and before transplantation. Three blood samples from each patient, representing a maximum time span of 34 months, were analyzed. Levels of IgG, IgM isohemagglutinins, and antitetanus antibody were used as indicators of B-cell function. There were no significant differences between the individual values of a single patient with regard to each parameter. However, Group II patients had elevated values of total IgG relative to Group I patients. Total serum IgG-subclass levels (IgG1, IgG2, IgG3, IgG4) were measured and the relationship between a specific IgG subclass and the PRA activity was determined. IgG1 values in Group II were higher than those found for Group I. The other IgG subclasses were all within normal levels and were not significantly different between Group I and Group II. When IgG-subclass typing of PRA was performed, IgG1 accounted for most of the activity in both groups and a fall in PRA-specific IgG1 was associated with the reduced PRA observed in Group I. The data indicate that humoral immunity, as reflected by total and specific immunoglobulin levels, is intact in general in the two groups of presensitized renal allograft candidates examined and that any loss of PRA activity reflects a reduction in a specific immune response.

AB - The influence of presensitization (blood transfusions) on B-cell immunity as reflected in the serum of two groups of candidates for cadaveric donor renal allografts was examined. The first group initially had a high level of panel-reactive antibody (PRA) greater than 72% but experienced a large decrease in PRA (>70%) 6-34 months prior to transplantation. In contrast, the second group maintained a high PRA (100%) for up to 28 months after sensitization and before transplantation. Three blood samples from each patient, representing a maximum time span of 34 months, were analyzed. Levels of IgG, IgM isohemagglutinins, and antitetanus antibody were used as indicators of B-cell function. There were no significant differences between the individual values of a single patient with regard to each parameter. However, Group II patients had elevated values of total IgG relative to Group I patients. Total serum IgG-subclass levels (IgG1, IgG2, IgG3, IgG4) were measured and the relationship between a specific IgG subclass and the PRA activity was determined. IgG1 values in Group II were higher than those found for Group I. The other IgG subclasses were all within normal levels and were not significantly different between Group I and Group II. When IgG-subclass typing of PRA was performed, IgG1 accounted for most of the activity in both groups and a fall in PRA-specific IgG1 was associated with the reduced PRA observed in Group I. The data indicate that humoral immunity, as reflected by total and specific immunoglobulin levels, is intact in general in the two groups of presensitized renal allograft candidates examined and that any loss of PRA activity reflects a reduction in a specific immune response.

KW - B-cell immunity

KW - IgG subclasses

KW - panel-reactive antibodies

KW - Pretransplant sensitization

UR - http://www.scopus.com/inward/record.url?scp=0023945224&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023945224&partnerID=8YFLogxK

U2 - 10.1007/BF00917567

DO - 10.1007/BF00917567

M3 - Article

C2 - 3292565

AN - SCOPUS:0023945224

VL - 8

SP - 200

EP - 206

JO - Journal of Clinical Immunology

JF - Journal of Clinical Immunology

SN - 0271-9142

IS - 3

ER -