Epigenetic characterization of the CpG islands of bovine Leptin and POU5F1 genes in cloned bovine fetuses.

Maksym Kremenskoy, Yuliya Kremenska, Masako Suzuki, Kei Imai, Seiya Takahashi, Kazuyoshi Hashizume, Shintaro Yagi, Kunio Shiota

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Abnormal development and fetal loss during postimplantation period are concerns for production of nuclear transferred animals. Aberrant DNA methylation is one of the reasons for poor survival of cloned animals. In mammalian genome DNA, CpG islands are preferentially located at the start of transcription of housekeeping genes and are associated with tissue-specific genes. The correct and consecutive mechanisms of DNA methylation in the CpG islands are necessary for selective gene expressions that determine the properties of individual cells, tissues, and organs. In this study, we investigated the methylation status of the CpG islands of the bovine Leptin and POU5F1 genes in fetal and placental tissues from fetuses produced by artificial insemination (AI) and nuclear transfer (NT) at days 48 and 59 of pregnancy. Altered DNA methylation was observed in the normal and cloned fetal, placental, and endometrial tissues using bisulfite sequencing and pyrosequencing. Different tissue-specific methylated regions in the bovine Leptin and POU5F1 genes show a variable methylation status in NT fetuses compared to AI control.

Original languageEnglish (US)
Pages (from-to)277-285
Number of pages9
JournalThe Journal of reproduction and development
Volume52
Issue number2
DOIs
StatePublished - Apr 2006
Externally publishedYes

Fingerprint

CpG Islands
Leptin
leptin
Epigenomics
epigenetics
fetus
Fetus
DNA Methylation
DNA methylation
Artificial Insemination
cattle
Methylation
Genes
genes
artificial insemination
methylation
Essential Genes
Fetal Development
bisulfites
abnormal development

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Reproductive Medicine

Cite this

Epigenetic characterization of the CpG islands of bovine Leptin and POU5F1 genes in cloned bovine fetuses. / Kremenskoy, Maksym; Kremenska, Yuliya; Suzuki, Masako; Imai, Kei; Takahashi, Seiya; Hashizume, Kazuyoshi; Yagi, Shintaro; Shiota, Kunio.

In: The Journal of reproduction and development, Vol. 52, No. 2, 04.2006, p. 277-285.

Research output: Contribution to journalArticle

Kremenskoy, Maksym ; Kremenska, Yuliya ; Suzuki, Masako ; Imai, Kei ; Takahashi, Seiya ; Hashizume, Kazuyoshi ; Yagi, Shintaro ; Shiota, Kunio. / Epigenetic characterization of the CpG islands of bovine Leptin and POU5F1 genes in cloned bovine fetuses. In: The Journal of reproduction and development. 2006 ; Vol. 52, No. 2. pp. 277-285.
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