EphB controls NMDA receptor function and synaptic targeting in a subunit-specific manner

Mark J. Nolt, Ying Lin, Martin Hruska, Jessica Murphy, Sean I. Sheffler-Colins, Matthew S. Kayser, Joel Passer, Michael V. L. Bennett, R. Suzanne Zukin, Matthew B. Dalva

Research output: Contribution to journalArticle

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Abstract

Dynamic regulation of the localization and function of NMDA receptors (NMDARs) is critical for synaptic development and function. The composition and localization of NMDAR subunits at synapses are tightly regulated and can influence the ability of individual synapses to undergo long-lasting changes in response to stimuli. Here, we examine mechanisms by which EphB2, a receptor tyrosine kinase that binds and phosphorylates NMDA Rs, controls NMDAR subunit localization and function at synapses. We find that, in mature neurons, EphB2 expression levels regulate the amount of NMDAR sat synapses, and Eph B activation decreases Ca2+-dependent desensitization of NR2B-containing NMDA Rs. Eph Bs are required for enhanced localization of NR2B-containing NMDARs at synapses of mature neurons; triple Eph B knock-out mice lacking EphB1-3 exhibit homeostatic up regulation of NMDAR surface expression and loss of proper targeting to synaptic sites. These findings demonstrate that, in the mature nervous system, EphBs are key regulators of the synaptic localization of NMDA Rs.

Original languageEnglish (US)
Pages (from-to)5353-5364
Number of pages12
JournalJournal of Neuroscience
Volume31
Issue number14
DOIs
StatePublished - Apr 6 2011

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N-Methyl-D-Aspartate Receptors
Synapses
N-Methylaspartate
EphB2 Receptors
Neurons
Knockout Mice
Protein-Tyrosine Kinases
Nervous System
Up-Regulation

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Nolt, M. J., Lin, Y., Hruska, M., Murphy, J., Sheffler-Colins, S. I., Kayser, M. S., ... Dalva, M. B. (2011). EphB controls NMDA receptor function and synaptic targeting in a subunit-specific manner. Journal of Neuroscience, 31(14), 5353-5364. https://doi.org/10.1523/JNEUROSCI.0282-11.2011

EphB controls NMDA receptor function and synaptic targeting in a subunit-specific manner. / Nolt, Mark J.; Lin, Ying; Hruska, Martin; Murphy, Jessica; Sheffler-Colins, Sean I.; Kayser, Matthew S.; Passer, Joel; Bennett, Michael V. L.; Zukin, R. Suzanne; Dalva, Matthew B.

In: Journal of Neuroscience, Vol. 31, No. 14, 06.04.2011, p. 5353-5364.

Research output: Contribution to journalArticle

Nolt, MJ, Lin, Y, Hruska, M, Murphy, J, Sheffler-Colins, SI, Kayser, MS, Passer, J, Bennett, MVL, Zukin, RS & Dalva, MB 2011, 'EphB controls NMDA receptor function and synaptic targeting in a subunit-specific manner', Journal of Neuroscience, vol. 31, no. 14, pp. 5353-5364. https://doi.org/10.1523/JNEUROSCI.0282-11.2011
Nolt MJ, Lin Y, Hruska M, Murphy J, Sheffler-Colins SI, Kayser MS et al. EphB controls NMDA receptor function and synaptic targeting in a subunit-specific manner. Journal of Neuroscience. 2011 Apr 6;31(14):5353-5364. https://doi.org/10.1523/JNEUROSCI.0282-11.2011
Nolt, Mark J. ; Lin, Ying ; Hruska, Martin ; Murphy, Jessica ; Sheffler-Colins, Sean I. ; Kayser, Matthew S. ; Passer, Joel ; Bennett, Michael V. L. ; Zukin, R. Suzanne ; Dalva, Matthew B. / EphB controls NMDA receptor function and synaptic targeting in a subunit-specific manner. In: Journal of Neuroscience. 2011 ; Vol. 31, No. 14. pp. 5353-5364.
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