Enzymic synthesis of deoxyribonucleic acid: XVII. Some unusual physical properties of the product primed by native DNA templates

Carl L. Schildkraut, Charles C. Richardson, Arthur Kornberg

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Abstract

Extensive replication of a helical DNA produces a complex of primer and product which can be dissociated by denaturing treatments. The physical characteristics of the enzymic product resemble those of a native DNA except in two ways: (1) an unusual capacity to resume a helical conformation after denaturing treatments (“non-denaturability”); and (2) appearance of a branched structure in the electron microscope. Exposure of the enzymic product to extensive protease, mild nuclease or prolonged heating treatment fails to alter its “non-denaturability”. These and other studies suggest that in the replication of native DNA the new strands are not covalently linked to the primer and that a “multiple hairpin” or “pleated” structure may develop which recovers considerable helicity following denaturing treatments. By contrast, these and other studies (described by Richardson, Inman & Kornberg, 1964a) also indicate that in the repair of partially single-stranded DNA, the new strands are covalently linked to the primer and appear to have all the physical properties of naturally occurring DNA.

Original languageEnglish (US)
Pages (from-to)24-45
Number of pages22
JournalJournal of Molecular Biology
Volume9
Issue number1
DOIs
Publication statusPublished - Jan 1 1964
Externally publishedYes

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ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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