Enzymatic differences between chronic lymphocytic leukemia and prolymphocytic leukemia

Howard Ratech, William Z. Borer, Carl D. Winberg, Henry Rappaport

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Chronic lymphocytic leukemia and prolymphocytic leukemia of the B-cell immunophenotype are closely related disorders, but differ in their cytomorphologic and clinical features. In an attempt to differentiate further between these two forms of leukemia, we measured adenosine deaminase and purine nucleoside phosphorylase activities by using a linked-enzyme spectrophotometric assay on peripheral-blood leukemic cells from seven patients with chronic lymphocytic leukemia, three patients with prolymphocytic leukemia, and one patient with prolymphocytoid transformation of chronic lymphocytic leukemia. By using discriminant analysis, we were able to distinguish the two groups only on the basis of purine nucleoside phosphorylase activity (F1,9; p<0.001). The purine nucleoside phosphorylase activity in leukemic cells with prolymphocytic cytomorphology was significantly elevated (mean = 58.6 nM/min/mg protein) compared to the activity in leukemic cells with lymphocytic cytomorphology (mean = 25.6 nM/min/mg protein). There was only one patient with chronic lymphocytic leukemia who was assigned to the prolymphocytic leukemia group on the basis of her purine nucleoside phosphorylase activity. Our study suggests that purine nucleoside phosphorylase activity in leukemic cells may be useful in the distinction of prolymphocytic leukemia from chronic lymphocytic leukemia, and that it may be an enzymatic marker for the early detection of prolymphocytoid transformation of chronic lymphocytic leukemia.

Original languageEnglish (US)
Pages (from-to)1271-1275
Number of pages5
JournalLeukemia Research
Volume9
Issue number10
DOIs
StatePublished - 1985
Externally publishedYes

Fingerprint

Prolymphocytic Leukemia
Purine-Nucleoside Phosphorylase
B-Cell Chronic Lymphocytic Leukemia
B-Cell Prolymphocytic Leukemia
Adenosine Deaminase
Enzyme Assays
Discriminant Analysis
Blood Cells
Leukemia
Proteins

Keywords

  • Adenosine deaminase
  • lymphocytic leukemia
  • purine nucleoside phosphorylase

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

Cite this

Enzymatic differences between chronic lymphocytic leukemia and prolymphocytic leukemia. / Ratech, Howard; Borer, William Z.; Winberg, Carl D.; Rappaport, Henry.

In: Leukemia Research, Vol. 9, No. 10, 1985, p. 1271-1275.

Research output: Contribution to journalArticle

Ratech, Howard ; Borer, William Z. ; Winberg, Carl D. ; Rappaport, Henry. / Enzymatic differences between chronic lymphocytic leukemia and prolymphocytic leukemia. In: Leukemia Research. 1985 ; Vol. 9, No. 10. pp. 1271-1275.
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