Enhanced enzyme-linked immunosorbent assay for detection of antibodies to virus-like particles of human papillomavirus

Yevgeniy Y. Studentsov, Mark Schiffman, Howard Strickler, Gloria Y F Ho, Yuk Ying Susana Pang, John Schiller, Rolando Herrero, Robert D. Burk

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Measurement of antibodies to human papillomavirus (HPV) is complicated by many factors. Although enzyme-linked immunosorbent assays (ELISAs) that use virus-like particles (VLPs) have proved useful, the assays have, in general, had moderate sensitivities and low signal-to-noise ratios. To enhance the performance of the assay, a systematic investigation was undertaken to examine key variables used in ELISAs for the detection of antibodies to VLPs of HPV. Incorporation of two vinyl polymers, polyvinyl alcohol (molecular weight, 50,000) (PVA-50) and polyvinylpyrrolidone (molecular weight, 360,000) (PVP-360), was found to increase the sensitivity as well as the specificity of the assay for the detection of antibodies to VLPs of HPV. In particular, the addition of PVA-50 to the blocking solution reduced the amount of nonspecific binding of antibodies to VLPs and the microplate surface, whereas the addition of PVP-360 increased the sensitivity of antibody detection. The new ELISA demonstrated increased sensitivity and specificity for the detection of cervical HPV type 16 infection compared to those of a prototype assay with coded clinical serum samples from women with known cervicovaginal HPV infection status. It is anticipated that the enhanced ELISA conditions will have wide application to a large number of clinical diagnostic assays.

Original languageEnglish (US)
Pages (from-to)1755-1760
Number of pages6
JournalJournal of Clinical Microbiology
Volume40
Issue number5
DOIs
StatePublished - 2002

Fingerprint

Virion
Enzyme-Linked Immunosorbent Assay
Antibodies
Papillomavirus Infections
Molecular Weight
Polyvinyl Alcohol
Povidone
Human papillomavirus 16
Signal-To-Noise Ratio
Polymers
Sensitivity and Specificity
Serum

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Enhanced enzyme-linked immunosorbent assay for detection of antibodies to virus-like particles of human papillomavirus. / Studentsov, Yevgeniy Y.; Schiffman, Mark; Strickler, Howard; Ho, Gloria Y F; Susana Pang, Yuk Ying; Schiller, John; Herrero, Rolando; Burk, Robert D.

In: Journal of Clinical Microbiology, Vol. 40, No. 5, 2002, p. 1755-1760.

Research output: Contribution to journalArticle

Studentsov, Yevgeniy Y. ; Schiffman, Mark ; Strickler, Howard ; Ho, Gloria Y F ; Susana Pang, Yuk Ying ; Schiller, John ; Herrero, Rolando ; Burk, Robert D. / Enhanced enzyme-linked immunosorbent assay for detection of antibodies to virus-like particles of human papillomavirus. In: Journal of Clinical Microbiology. 2002 ; Vol. 40, No. 5. pp. 1755-1760.
@article{d32be3dddfbd46bca60d6ed9228b418a,
title = "Enhanced enzyme-linked immunosorbent assay for detection of antibodies to virus-like particles of human papillomavirus",
abstract = "Measurement of antibodies to human papillomavirus (HPV) is complicated by many factors. Although enzyme-linked immunosorbent assays (ELISAs) that use virus-like particles (VLPs) have proved useful, the assays have, in general, had moderate sensitivities and low signal-to-noise ratios. To enhance the performance of the assay, a systematic investigation was undertaken to examine key variables used in ELISAs for the detection of antibodies to VLPs of HPV. Incorporation of two vinyl polymers, polyvinyl alcohol (molecular weight, 50,000) (PVA-50) and polyvinylpyrrolidone (molecular weight, 360,000) (PVP-360), was found to increase the sensitivity as well as the specificity of the assay for the detection of antibodies to VLPs of HPV. In particular, the addition of PVA-50 to the blocking solution reduced the amount of nonspecific binding of antibodies to VLPs and the microplate surface, whereas the addition of PVP-360 increased the sensitivity of antibody detection. The new ELISA demonstrated increased sensitivity and specificity for the detection of cervical HPV type 16 infection compared to those of a prototype assay with coded clinical serum samples from women with known cervicovaginal HPV infection status. It is anticipated that the enhanced ELISA conditions will have wide application to a large number of clinical diagnostic assays.",
author = "Studentsov, {Yevgeniy Y.} and Mark Schiffman and Howard Strickler and Ho, {Gloria Y F} and {Susana Pang}, {Yuk Ying} and John Schiller and Rolando Herrero and Burk, {Robert D.}",
year = "2002",
doi = "10.1128/JCM.40.5.1755-1760.2002",
language = "English (US)",
volume = "40",
pages = "1755--1760",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "5",

}

TY - JOUR

T1 - Enhanced enzyme-linked immunosorbent assay for detection of antibodies to virus-like particles of human papillomavirus

AU - Studentsov, Yevgeniy Y.

AU - Schiffman, Mark

AU - Strickler, Howard

AU - Ho, Gloria Y F

AU - Susana Pang, Yuk Ying

AU - Schiller, John

AU - Herrero, Rolando

AU - Burk, Robert D.

PY - 2002

Y1 - 2002

N2 - Measurement of antibodies to human papillomavirus (HPV) is complicated by many factors. Although enzyme-linked immunosorbent assays (ELISAs) that use virus-like particles (VLPs) have proved useful, the assays have, in general, had moderate sensitivities and low signal-to-noise ratios. To enhance the performance of the assay, a systematic investigation was undertaken to examine key variables used in ELISAs for the detection of antibodies to VLPs of HPV. Incorporation of two vinyl polymers, polyvinyl alcohol (molecular weight, 50,000) (PVA-50) and polyvinylpyrrolidone (molecular weight, 360,000) (PVP-360), was found to increase the sensitivity as well as the specificity of the assay for the detection of antibodies to VLPs of HPV. In particular, the addition of PVA-50 to the blocking solution reduced the amount of nonspecific binding of antibodies to VLPs and the microplate surface, whereas the addition of PVP-360 increased the sensitivity of antibody detection. The new ELISA demonstrated increased sensitivity and specificity for the detection of cervical HPV type 16 infection compared to those of a prototype assay with coded clinical serum samples from women with known cervicovaginal HPV infection status. It is anticipated that the enhanced ELISA conditions will have wide application to a large number of clinical diagnostic assays.

AB - Measurement of antibodies to human papillomavirus (HPV) is complicated by many factors. Although enzyme-linked immunosorbent assays (ELISAs) that use virus-like particles (VLPs) have proved useful, the assays have, in general, had moderate sensitivities and low signal-to-noise ratios. To enhance the performance of the assay, a systematic investigation was undertaken to examine key variables used in ELISAs for the detection of antibodies to VLPs of HPV. Incorporation of two vinyl polymers, polyvinyl alcohol (molecular weight, 50,000) (PVA-50) and polyvinylpyrrolidone (molecular weight, 360,000) (PVP-360), was found to increase the sensitivity as well as the specificity of the assay for the detection of antibodies to VLPs of HPV. In particular, the addition of PVA-50 to the blocking solution reduced the amount of nonspecific binding of antibodies to VLPs and the microplate surface, whereas the addition of PVP-360 increased the sensitivity of antibody detection. The new ELISA demonstrated increased sensitivity and specificity for the detection of cervical HPV type 16 infection compared to those of a prototype assay with coded clinical serum samples from women with known cervicovaginal HPV infection status. It is anticipated that the enhanced ELISA conditions will have wide application to a large number of clinical diagnostic assays.

UR - http://www.scopus.com/inward/record.url?scp=0036247240&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036247240&partnerID=8YFLogxK

U2 - 10.1128/JCM.40.5.1755-1760.2002

DO - 10.1128/JCM.40.5.1755-1760.2002

M3 - Article

VL - 40

SP - 1755

EP - 1760

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 5

ER -