Electrophysiological analysis of factors involved in the primary demyelinating diseases: the rabbit eye model system

Joseph C. Arezzo, Celia F. Brosnan, Charles E. Schroeder, Mona S. Litwak, Murray B. Bornstein

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

This study explores the longitudinal assessment of visual evoked potentials (VEPs) in the rabbit as a method for defining factors underlying functional and structural changes associated with optic neuritis and the inflammatory demyelinating diseases. In rabbits with experimental autoimmune encephalomyelitis (EAE) induced by sensitization with guinea pig spinal cord myelin, injection of lymphokines into the posterior chamber of one eye (monocular challenge) produces an early inflammatory response in the retina and optic nerve, and an alteration in the VEP, all limited to the injected eye and its projections. The earliest changes in the timing and distribution of the cortical VEP occur within hours of ocular challenge and precede histopathological evidence of structural demyelination at the light microscope level. Prechallenge assessment allows the induced monocular prechiasmal effects to be distinguished from the more diffuse electrophysiological findings associated with EAE (i.e. those due to sensitization alone). In sensitized/challenged animals there is a clear correspondence between electrophysiological and morphological measures of dysfunction at the time points sampled. These results suggest that this model system affords an excellent opportunity to examine the precise structural correlates of the early functional changes associated with the onset of inflammatory demyelination within the CNS. Furthermore, the stability of the system provides the capacity to monitor alterations over the complete course of inflammation, demyelination and remyelination, induced by experimental manipulations.

Original languageEnglish (US)
Pages (from-to)286-300
Number of pages15
JournalBrain Research
Volume462
Issue number2
DOIs
StatePublished - Oct 18 1988

Fingerprint

Demyelinating Diseases
Statistical Factor Analysis
Visual Evoked Potentials
Rabbits
Autoimmune Experimental Encephalomyelitis
Optic Neuritis
Lymphokines
Optic Nerve
Myelin Sheath
Longitudinal Studies
Retina
Spinal Cord
Guinea Pigs
Inflammation
Light
Injections

Keywords

  • Demyelination
  • Experimental autoimmune encephalomyelitis
  • Inflammation
  • Rabbit
  • Visual evoked potential

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Electrophysiological analysis of factors involved in the primary demyelinating diseases : the rabbit eye model system. / Arezzo, Joseph C.; Brosnan, Celia F.; Schroeder, Charles E.; Litwak, Mona S.; Bornstein, Murray B.

In: Brain Research, Vol. 462, No. 2, 18.10.1988, p. 286-300.

Research output: Contribution to journalArticle

Arezzo, Joseph C. ; Brosnan, Celia F. ; Schroeder, Charles E. ; Litwak, Mona S. ; Bornstein, Murray B. / Electrophysiological analysis of factors involved in the primary demyelinating diseases : the rabbit eye model system. In: Brain Research. 1988 ; Vol. 462, No. 2. pp. 286-300.
@article{2452b48a334c4e18969c334701f2031b,
title = "Electrophysiological analysis of factors involved in the primary demyelinating diseases: the rabbit eye model system",
abstract = "This study explores the longitudinal assessment of visual evoked potentials (VEPs) in the rabbit as a method for defining factors underlying functional and structural changes associated with optic neuritis and the inflammatory demyelinating diseases. In rabbits with experimental autoimmune encephalomyelitis (EAE) induced by sensitization with guinea pig spinal cord myelin, injection of lymphokines into the posterior chamber of one eye (monocular challenge) produces an early inflammatory response in the retina and optic nerve, and an alteration in the VEP, all limited to the injected eye and its projections. The earliest changes in the timing and distribution of the cortical VEP occur within hours of ocular challenge and precede histopathological evidence of structural demyelination at the light microscope level. Prechallenge assessment allows the induced monocular prechiasmal effects to be distinguished from the more diffuse electrophysiological findings associated with EAE (i.e. those due to sensitization alone). In sensitized/challenged animals there is a clear correspondence between electrophysiological and morphological measures of dysfunction at the time points sampled. These results suggest that this model system affords an excellent opportunity to examine the precise structural correlates of the early functional changes associated with the onset of inflammatory demyelination within the CNS. Furthermore, the stability of the system provides the capacity to monitor alterations over the complete course of inflammation, demyelination and remyelination, induced by experimental manipulations.",
keywords = "Demyelination, Experimental autoimmune encephalomyelitis, Inflammation, Rabbit, Visual evoked potential",
author = "Arezzo, {Joseph C.} and Brosnan, {Celia F.} and Schroeder, {Charles E.} and Litwak, {Mona S.} and Bornstein, {Murray B.}",
year = "1988",
month = "10",
day = "18",
doi = "10.1016/0006-8993(88)90557-4",
language = "English (US)",
volume = "462",
pages = "286--300",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Electrophysiological analysis of factors involved in the primary demyelinating diseases

T2 - the rabbit eye model system

AU - Arezzo, Joseph C.

AU - Brosnan, Celia F.

AU - Schroeder, Charles E.

AU - Litwak, Mona S.

AU - Bornstein, Murray B.

PY - 1988/10/18

Y1 - 1988/10/18

N2 - This study explores the longitudinal assessment of visual evoked potentials (VEPs) in the rabbit as a method for defining factors underlying functional and structural changes associated with optic neuritis and the inflammatory demyelinating diseases. In rabbits with experimental autoimmune encephalomyelitis (EAE) induced by sensitization with guinea pig spinal cord myelin, injection of lymphokines into the posterior chamber of one eye (monocular challenge) produces an early inflammatory response in the retina and optic nerve, and an alteration in the VEP, all limited to the injected eye and its projections. The earliest changes in the timing and distribution of the cortical VEP occur within hours of ocular challenge and precede histopathological evidence of structural demyelination at the light microscope level. Prechallenge assessment allows the induced monocular prechiasmal effects to be distinguished from the more diffuse electrophysiological findings associated with EAE (i.e. those due to sensitization alone). In sensitized/challenged animals there is a clear correspondence between electrophysiological and morphological measures of dysfunction at the time points sampled. These results suggest that this model system affords an excellent opportunity to examine the precise structural correlates of the early functional changes associated with the onset of inflammatory demyelination within the CNS. Furthermore, the stability of the system provides the capacity to monitor alterations over the complete course of inflammation, demyelination and remyelination, induced by experimental manipulations.

AB - This study explores the longitudinal assessment of visual evoked potentials (VEPs) in the rabbit as a method for defining factors underlying functional and structural changes associated with optic neuritis and the inflammatory demyelinating diseases. In rabbits with experimental autoimmune encephalomyelitis (EAE) induced by sensitization with guinea pig spinal cord myelin, injection of lymphokines into the posterior chamber of one eye (monocular challenge) produces an early inflammatory response in the retina and optic nerve, and an alteration in the VEP, all limited to the injected eye and its projections. The earliest changes in the timing and distribution of the cortical VEP occur within hours of ocular challenge and precede histopathological evidence of structural demyelination at the light microscope level. Prechallenge assessment allows the induced monocular prechiasmal effects to be distinguished from the more diffuse electrophysiological findings associated with EAE (i.e. those due to sensitization alone). In sensitized/challenged animals there is a clear correspondence between electrophysiological and morphological measures of dysfunction at the time points sampled. These results suggest that this model system affords an excellent opportunity to examine the precise structural correlates of the early functional changes associated with the onset of inflammatory demyelination within the CNS. Furthermore, the stability of the system provides the capacity to monitor alterations over the complete course of inflammation, demyelination and remyelination, induced by experimental manipulations.

KW - Demyelination

KW - Experimental autoimmune encephalomyelitis

KW - Inflammation

KW - Rabbit

KW - Visual evoked potential

UR - http://www.scopus.com/inward/record.url?scp=0023805783&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023805783&partnerID=8YFLogxK

U2 - 10.1016/0006-8993(88)90557-4

DO - 10.1016/0006-8993(88)90557-4

M3 - Article

C2 - 3191390

AN - SCOPUS:0023805783

VL - 462

SP - 286

EP - 300

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 2

ER -