Electron spin echo envelope modulation studies of lectins: Evidence for a conserved Mn2+-binding site

John McCracken, Jack Peisach, Lokesh Bhattacharyya, Curtis F. Brewer

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Electron spin echo envelope modulation (ESEEM) experiments have been used to investigate the Mn2+-binding site in a series of lectins including concanavalin A, pea lectin (Pisum sativum), isolectin A from lentil (Lens culinaris), soybean agglutinin (Glycine max), Erythrina indica lectin, and Lotus tetragonolobus isolectin A. Together with model studies, the results provide direct evidence for a single nitrogen atom of a conserved residue bonded directly to Mn2+ in all of them. ESEEM measurements of the lectins exchanged with deuterium oxide, together with model studies, provide evidence for the presence of two water molecules coordinated to the Mn2+ in all of the proteins. In contrast to concanavalin A, the absence of solvent exchange at the Mn2+ site in the pea and lentil lectins demonstrated by nuclear magnetic relaxation dispersion measurements [Bhattacharyya, L., Brewer, C. F., Brown, R. D., III, & Koenig, S. H. (1985) Biochemistry 24, 4985-4990] must therefore be due to slow exchange of the water ligands of the bound Mn2+. Binding of saccharides was observed to have little effect on the structural features of the Mn2+ site in the lectins as determined by ESEEM.

Original languageEnglish (US)
Pages (from-to)4486-4491
Number of pages6
JournalBiochemistry
Volume30
Issue number18
StatePublished - 1991

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Lectins
Binding Sites
Modulation
Electrons
Concanavalin A
Deuterium Oxide
Magnetic relaxation
Lens Plant
Biochemistry
Water
Peas
Soybeans
Glycine
Lenses
Nitrogen
Ligands
Atoms
Molecules
Proteins
Experiments

ASJC Scopus subject areas

  • Biochemistry

Cite this

Electron spin echo envelope modulation studies of lectins : Evidence for a conserved Mn2+-binding site. / McCracken, John; Peisach, Jack; Bhattacharyya, Lokesh; Brewer, Curtis F.

In: Biochemistry, Vol. 30, No. 18, 1991, p. 4486-4491.

Research output: Contribution to journalArticle

McCracken, J, Peisach, J, Bhattacharyya, L & Brewer, CF 1991, 'Electron spin echo envelope modulation studies of lectins: Evidence for a conserved Mn2+-binding site', Biochemistry, vol. 30, no. 18, pp. 4486-4491.
McCracken, John ; Peisach, Jack ; Bhattacharyya, Lokesh ; Brewer, Curtis F. / Electron spin echo envelope modulation studies of lectins : Evidence for a conserved Mn2+-binding site. In: Biochemistry. 1991 ; Vol. 30, No. 18. pp. 4486-4491.
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