Electron Spin Echo Envelope Modulation Studies of Lectins: Evidence for A Conserved Mn2+-Binding Site

John McCracken, Jack Peisach, Lokesh Bhattacharyya, Fred Brewer

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Electron spin echo envelope modulation (ESEEM) experiments have been used to investigate the Mn2+-binding site in a series of lectins including concanavalin A, pea lectin (Pisum satiυum), isolectin A from lentil (Lens culinaris), soybean agglutinin (Glycine max), Erythrina indica lectin, and Lotus tetragonolobus isolectin A. Together with model studies, the results provide direct evidence for a single nitrogen atom of a conserved residue bonded directly to Mn2+ in all of them. ESEEM measurements of the lectins exchanged with deuterium oxide, together with model studies, provide evidence for the presence of two water molecules coordinated to the Mn2+ in all of the proteins. In contrast to concanavalin A, the absence of solvent exchange at the Mn2+ site in the pea and lentil lectins demonstrated by nuclear magnetic relaxation dispersion measurements [Bhattacharyya, L., Brewer, C. F., Brown, R. D., III, & Koenig, S. H. (1985) Biochemistry 24, 4985–4990] must therefore be due to slow exchange of the water ligands of the bound Mn2+. Binding of saccharides was observed to have little effect on the structural features of the Mn2+ site in the lectins as determined by ESEEM.

Original languageEnglish (US)
Pages (from-to)4486-4491
Number of pages6
JournalBiochemistry
Volume30
Issue number18
DOIs
StatePublished - May 1 1991

ASJC Scopus subject areas

  • Biochemistry

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