Electrical coupling between cells of the insect Aedes albopictus

F. Bukauskas, C. Kempf, R. Weingart

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

1. Cell pairs of an insect cell line (Aedes albopictus, clone C6/36) were used to study the electrical properties of intercellular junctions. A double voltage-clamp approach was adopted to control the voltage gradient between the cells and measure the intracellular current flow. 2. Determinations of junctional conductance (g(j)) revealed two types of intercellular contacts, gap junctions and cytoplasmic bridges. Identification occurred by means of functional criteria, i.e. the dependency of g(j) on (i) junctional membrane potential, (ii) non-junctional membrane potential, and (iii) heptanol. 3. In cell pairs with putative gap junctions, g(j) was dependent on the junctional membrane potential (V(j)). When determined at the beginning of voltage pulses, g(j) was insensitive to V(j): when determined at the end of 15 s pulses, it depended on V(j) in a bell-shaped manner (70% decrease for a change in V(j) of ±75mV). 4. These cell pairs also showed a dependency of g(j) on the non-junctional membrane potential (Vm). When determined immediately after changing the non-junctional membrane potential in both cells, g(j) was not affected by V(m); when determined 30 s later, g(j) was modified by V(m), in a S-shaped fashion (100% decrease when V(m) was depolarized to +50mV). 5. Exposure to 3 mM heptanol gave rise to complete and reversible block of g(j) in cell pairs with putative gap junctions. 6. Cell pairs susceptible to uncoupling by heptanol revealed junctional currents indicative of the operation of gap junction channels. The single-channel conductance, determined at a V(m) of -50 to -70mV, was 133 pS. 7. In the case of putative cytoplasmic bridges, g(j) was insensitive to the junctional and non-junctional membrane potential. In addition, it was not affected by 3 mM-heptanol. 8. While most cell pairs showed functional properties characteristic of gap junctions or cytoplasmic bridges, few cell pairs exhibited junctional currents compatible with the co-existence of both junctional structures.

Original languageEnglish (US)
Pages (from-to)321-337
Number of pages17
JournalJournal of Physiology
Volume448
StatePublished - 1992
Externally publishedYes

Fingerprint

Aedes
Insects
Heptanol
Gap Junctions
Membrane Potentials
Intercellular Junctions
Clone Cells
Cell Line

ASJC Scopus subject areas

  • Physiology

Cite this

Bukauskas, F., Kempf, C., & Weingart, R. (1992). Electrical coupling between cells of the insect Aedes albopictus. Journal of Physiology, 448, 321-337.

Electrical coupling between cells of the insect Aedes albopictus. / Bukauskas, F.; Kempf, C.; Weingart, R.

In: Journal of Physiology, Vol. 448, 1992, p. 321-337.

Research output: Contribution to journalArticle

Bukauskas, F, Kempf, C & Weingart, R 1992, 'Electrical coupling between cells of the insect Aedes albopictus', Journal of Physiology, vol. 448, pp. 321-337.
Bukauskas, F. ; Kempf, C. ; Weingart, R. / Electrical coupling between cells of the insect Aedes albopictus. In: Journal of Physiology. 1992 ; Vol. 448. pp. 321-337.
@article{fc1016a5ded549b8841a46d19a68766a,
title = "Electrical coupling between cells of the insect Aedes albopictus",
abstract = "1. Cell pairs of an insect cell line (Aedes albopictus, clone C6/36) were used to study the electrical properties of intercellular junctions. A double voltage-clamp approach was adopted to control the voltage gradient between the cells and measure the intracellular current flow. 2. Determinations of junctional conductance (g(j)) revealed two types of intercellular contacts, gap junctions and cytoplasmic bridges. Identification occurred by means of functional criteria, i.e. the dependency of g(j) on (i) junctional membrane potential, (ii) non-junctional membrane potential, and (iii) heptanol. 3. In cell pairs with putative gap junctions, g(j) was dependent on the junctional membrane potential (V(j)). When determined at the beginning of voltage pulses, g(j) was insensitive to V(j): when determined at the end of 15 s pulses, it depended on V(j) in a bell-shaped manner (70{\%} decrease for a change in V(j) of ±75mV). 4. These cell pairs also showed a dependency of g(j) on the non-junctional membrane potential (Vm). When determined immediately after changing the non-junctional membrane potential in both cells, g(j) was not affected by V(m); when determined 30 s later, g(j) was modified by V(m), in a S-shaped fashion (100{\%} decrease when V(m) was depolarized to +50mV). 5. Exposure to 3 mM heptanol gave rise to complete and reversible block of g(j) in cell pairs with putative gap junctions. 6. Cell pairs susceptible to uncoupling by heptanol revealed junctional currents indicative of the operation of gap junction channels. The single-channel conductance, determined at a V(m) of -50 to -70mV, was 133 pS. 7. In the case of putative cytoplasmic bridges, g(j) was insensitive to the junctional and non-junctional membrane potential. In addition, it was not affected by 3 mM-heptanol. 8. While most cell pairs showed functional properties characteristic of gap junctions or cytoplasmic bridges, few cell pairs exhibited junctional currents compatible with the co-existence of both junctional structures.",
author = "F. Bukauskas and C. Kempf and R. Weingart",
year = "1992",
language = "English (US)",
volume = "448",
pages = "321--337",
journal = "Journal of Physiology",
issn = "0022-3751",
publisher = "Wiley-Blackwell",

}

TY - JOUR

T1 - Electrical coupling between cells of the insect Aedes albopictus

AU - Bukauskas, F.

AU - Kempf, C.

AU - Weingart, R.

PY - 1992

Y1 - 1992

N2 - 1. Cell pairs of an insect cell line (Aedes albopictus, clone C6/36) were used to study the electrical properties of intercellular junctions. A double voltage-clamp approach was adopted to control the voltage gradient between the cells and measure the intracellular current flow. 2. Determinations of junctional conductance (g(j)) revealed two types of intercellular contacts, gap junctions and cytoplasmic bridges. Identification occurred by means of functional criteria, i.e. the dependency of g(j) on (i) junctional membrane potential, (ii) non-junctional membrane potential, and (iii) heptanol. 3. In cell pairs with putative gap junctions, g(j) was dependent on the junctional membrane potential (V(j)). When determined at the beginning of voltage pulses, g(j) was insensitive to V(j): when determined at the end of 15 s pulses, it depended on V(j) in a bell-shaped manner (70% decrease for a change in V(j) of ±75mV). 4. These cell pairs also showed a dependency of g(j) on the non-junctional membrane potential (Vm). When determined immediately after changing the non-junctional membrane potential in both cells, g(j) was not affected by V(m); when determined 30 s later, g(j) was modified by V(m), in a S-shaped fashion (100% decrease when V(m) was depolarized to +50mV). 5. Exposure to 3 mM heptanol gave rise to complete and reversible block of g(j) in cell pairs with putative gap junctions. 6. Cell pairs susceptible to uncoupling by heptanol revealed junctional currents indicative of the operation of gap junction channels. The single-channel conductance, determined at a V(m) of -50 to -70mV, was 133 pS. 7. In the case of putative cytoplasmic bridges, g(j) was insensitive to the junctional and non-junctional membrane potential. In addition, it was not affected by 3 mM-heptanol. 8. While most cell pairs showed functional properties characteristic of gap junctions or cytoplasmic bridges, few cell pairs exhibited junctional currents compatible with the co-existence of both junctional structures.

AB - 1. Cell pairs of an insect cell line (Aedes albopictus, clone C6/36) were used to study the electrical properties of intercellular junctions. A double voltage-clamp approach was adopted to control the voltage gradient between the cells and measure the intracellular current flow. 2. Determinations of junctional conductance (g(j)) revealed two types of intercellular contacts, gap junctions and cytoplasmic bridges. Identification occurred by means of functional criteria, i.e. the dependency of g(j) on (i) junctional membrane potential, (ii) non-junctional membrane potential, and (iii) heptanol. 3. In cell pairs with putative gap junctions, g(j) was dependent on the junctional membrane potential (V(j)). When determined at the beginning of voltage pulses, g(j) was insensitive to V(j): when determined at the end of 15 s pulses, it depended on V(j) in a bell-shaped manner (70% decrease for a change in V(j) of ±75mV). 4. These cell pairs also showed a dependency of g(j) on the non-junctional membrane potential (Vm). When determined immediately after changing the non-junctional membrane potential in both cells, g(j) was not affected by V(m); when determined 30 s later, g(j) was modified by V(m), in a S-shaped fashion (100% decrease when V(m) was depolarized to +50mV). 5. Exposure to 3 mM heptanol gave rise to complete and reversible block of g(j) in cell pairs with putative gap junctions. 6. Cell pairs susceptible to uncoupling by heptanol revealed junctional currents indicative of the operation of gap junction channels. The single-channel conductance, determined at a V(m) of -50 to -70mV, was 133 pS. 7. In the case of putative cytoplasmic bridges, g(j) was insensitive to the junctional and non-junctional membrane potential. In addition, it was not affected by 3 mM-heptanol. 8. While most cell pairs showed functional properties characteristic of gap junctions or cytoplasmic bridges, few cell pairs exhibited junctional currents compatible with the co-existence of both junctional structures.

UR - http://www.scopus.com/inward/record.url?scp=0026550102&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026550102&partnerID=8YFLogxK

M3 - Article

C2 - 1593470

AN - SCOPUS:0026550102

VL - 448

SP - 321

EP - 337

JO - Journal of Physiology

JF - Journal of Physiology

SN - 0022-3751

ER -