Effects of estrogen on glucose uptake by rat muscle

Harry Shamoon, P. Felig

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The isolated rat diaphragm was used to study the effects of 17β estradiol on basal and insulin mediated glucose uptake. Rats were injected with estradiol for 2 wk in daily doses of 10 μg/100 g of body weight and were compared to untreated control animals. Estrogen treatment resulted in a 16% decrease in basal glucose uptake by diaphragm muscle as compared to controls. In contrast, in the presence of insulin, glucose uptake by muscle increased 103% above basal in estradiol treated animals as compared to a 38% rise in the control group. The absolute rate of glucose uptake induced by insulin in the estradiol treated animals (5.8 mg/g/hr) was 22% higher than in controls. These findings were not accompanied by changes in weight gain, plasma glucose and plasma immunoreactive insulin concentrations in the treated animals. In vitro incubation of diaphragm muscle with estradiol did not have an effect on basal or insulin mediated glucose uptake. The data indicate that treatment with naturally occurring estrogens increases muscle sensitivity to insulin stimulated glucose uptake. These findings suggest that the carbohydrate intolerance associated with the administration of oral contraceptives may be related to the use of synthetic rather than natural estrogens and/or progestins in such preparations.

Original languageEnglish (US)
Pages (from-to)227-233
Number of pages7
JournalYale Journal of Biology and Medicine
Volume47
Issue number4
StatePublished - 1974
Externally publishedYes

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Muscle
Rats
Estrogens
Glucose
Muscles
Estradiol
Insulin
Animals
Diaphragms
Diaphragm
Plasmas
Progestins
Oral Contraceptives
Weight Gain
Insulin Resistance
Body Weight
Carbohydrates
Control Groups

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Effects of estrogen on glucose uptake by rat muscle. / Shamoon, Harry; Felig, P.

In: Yale Journal of Biology and Medicine, Vol. 47, No. 4, 1974, p. 227-233.

Research output: Contribution to journalArticle

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