Effects of acrylamide on primary neonatal rat astrocyte functions

Michael Aschner, Qi Wu, Marvin A. Friedman

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The present study assessed biochemical endpoints indicative of acrylamide toxicity in astrocyte cultures derived from neonatal rat pups. Given earlier reports on the possible ability of acrylamide to induce astrocytomas in the Fischer 344 rat, we performed studies in neonatal rat astrocyte cultures from the Fischer 344 to assess the ability of acrylamide to induce astrocytic proliferation. Measurements on astrocytic proliferation included [ 3H]-leucine incorporation, [3H]-thymidine incorporation, and changes in proliferating cell nuclear antigen (PCNA). Although acrylamide (0.1 and 1 mM for 7, 11, 15, or 20 days) did not significantly (P > 0.05) affect [3H]-leucine or [3H]-thymidine incorporation, it significantly (F < 0.05) increased PCNA protein expression in astrocytes exposed to acrylamide for 15 and 20 days. Additional studies revealed that this effect on PCNA protein expression was not associated with activation of dopamine-2 (D2) receptors, given that quinpirole (10 μM added to cultures for the last hour of 7, 11, 15, or 20 days in culture), a selective D2 receptor agonist, did not produce results analogous to those seen with acrylamide treatment. Cotreatment of astrocytes with acrylamide (7, 11, 15, or 20 days) aad the D2 receptor antagonist, sulpiride (1 μM for the last 6 h of exposure), also failed to reverse acrylamide's effect on PCNA protein induction. Taken together, these studies suggest that acrylamide promotes astrocytic cell proliferation in the CNS even though DNA synthesis did not appear stimulated.

Original languageEnglish (US)
Pages (from-to)444-454
Number of pages11
JournalAnnals of the New York Academy of Sciences
Volume1053
DOIs
StatePublished - 2005
Externally publishedYes

Fingerprint

Acrylamide
Astrocytes
Rats
Proliferating Cell Nuclear Antigen
Nuclear Proteins
Dopamine
Leucine
Thymidine
Quinpirole
Sulpiride
Proteins
Dopamine Antagonists
Cells
Rat
Dopamine Agonists
Astrocytoma
Inbred F344 Rats
Dopamine Receptors
Cell proliferation
Toxicity

Keywords

  • Acrylamide
  • Astrocyte
  • Leucine
  • Neonatal rat
  • Sulpiride
  • Thymidine

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Effects of acrylamide on primary neonatal rat astrocyte functions. / Aschner, Michael; Wu, Qi; Friedman, Marvin A.

In: Annals of the New York Academy of Sciences, Vol. 1053, 2005, p. 444-454.

Research output: Contribution to journalArticle

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