Effect of nitric oxide on acanthamoeba castellanii

Bora Yim, Joo Hee Park, Hyejoong Jeong, Jinkee Hong, Martha Kim, Minwook Chang, Roy S. Chuck, Choul Yong Park

Research output: Contribution to journalArticle

Abstract

PURPOSE. Acanthamoeba keratitis is a well-known intractable corneal infectious disease. We investigated the anti-Acanthamoeba effect of exogenous nitric oxide (NO). METHODS. Acanthamoeba castellanii was axenically cultured and exposed to various concentrations of NO donors, such as sodium nitrite, sodium nitroprusside (SNP), and NO-releasing silica nanoparticles (coated in branched polyethylene imine, size:100 nm), for 1 to 7 days (sodium nitrite and SNP: 0, 0.1, 1, 10, 100, and 1000 μM; silica nanoparticles: 0, 6.25, 12.5, 25, 50, and 100 μg/mL). Human corneal epithelial cells (HCECs) were cultured and exposed to sodium nitrite, SNP (0, 0.1, 1, 10, 100, and 1000 μM), and silica nanoparticles for 1, 2, and 3 days. RESULTS. Sodium nitrite and SNP showed a dose-dependent inhibitory effect on A. castellanii viability. A more prominent inhibitory effect was observed with SNP (less than 10% of organisms survived at 7-day culture with 1000 μM) compared with sodium nitrite. However, more cytotoxicity on HCEC was observed with SNP. NO-releasing silica nanoparticles were successfully internalized into the amoebic cytoplasm and accumulated in large vacuoles. Although blank silica nanoparticles had no inhibitory effect on A. castellanii viability, NO-releasing silica nanoparticles showed a dose-dependent amoebicidal effect. Furthermore, no cystic transformation of A. castellanii was observed under a phase contrast microscope or transmission electron microscope after exogenous NO treatment. CONCLUSIONS. Our results demonstrated the anti-Acanthamoeba effect of exogenous NO. This finding suggests that NO-releasing drug platforms, including nano-carriers, can be a promising therapeutic strategy for Acanthamoeba keratitis.

Original languageEnglish (US)
Pages (from-to)3239-3248
Number of pages10
JournalInvestigative Ophthalmology and Visual Science
Volume59
Issue number8
DOIs
StatePublished - Jul 1 2018

Fingerprint

Acanthamoeba castellanii
Sodium Nitrite
Nitroprusside
Nitric Oxide
Silicon Dioxide
Nanoparticles
Acanthamoeba Keratitis
Acanthamoeba
Epithelial Cells
Corneal Diseases
Imines
Nitric Oxide Donors
Polyethylene
Vacuoles
Communicable Diseases
Cytoplasm
Electrons

Keywords

  • Acanthamoeba
  • Cornea
  • Keratitis
  • Nitric oxide
  • Silica nanoparticle

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Yim, B., Park, J. H., Jeong, H., Hong, J., Kim, M., Chang, M., ... Park, C. Y. (2018). Effect of nitric oxide on acanthamoeba castellanii. Investigative Ophthalmology and Visual Science, 59(8), 3239-3248. https://doi.org/10.1167/iovs.18-23786

Effect of nitric oxide on acanthamoeba castellanii. / Yim, Bora; Park, Joo Hee; Jeong, Hyejoong; Hong, Jinkee; Kim, Martha; Chang, Minwook; Chuck, Roy S.; Park, Choul Yong.

In: Investigative Ophthalmology and Visual Science, Vol. 59, No. 8, 01.07.2018, p. 3239-3248.

Research output: Contribution to journalArticle

Yim, B, Park, JH, Jeong, H, Hong, J, Kim, M, Chang, M, Chuck, RS & Park, CY 2018, 'Effect of nitric oxide on acanthamoeba castellanii', Investigative Ophthalmology and Visual Science, vol. 59, no. 8, pp. 3239-3248. https://doi.org/10.1167/iovs.18-23786
Yim, Bora ; Park, Joo Hee ; Jeong, Hyejoong ; Hong, Jinkee ; Kim, Martha ; Chang, Minwook ; Chuck, Roy S. ; Park, Choul Yong. / Effect of nitric oxide on acanthamoeba castellanii. In: Investigative Ophthalmology and Visual Science. 2018 ; Vol. 59, No. 8. pp. 3239-3248.
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abstract = "PURPOSE. Acanthamoeba keratitis is a well-known intractable corneal infectious disease. We investigated the anti-Acanthamoeba effect of exogenous nitric oxide (NO). METHODS. Acanthamoeba castellanii was axenically cultured and exposed to various concentrations of NO donors, such as sodium nitrite, sodium nitroprusside (SNP), and NO-releasing silica nanoparticles (coated in branched polyethylene imine, size:100 nm), for 1 to 7 days (sodium nitrite and SNP: 0, 0.1, 1, 10, 100, and 1000 μM; silica nanoparticles: 0, 6.25, 12.5, 25, 50, and 100 μg/mL). Human corneal epithelial cells (HCECs) were cultured and exposed to sodium nitrite, SNP (0, 0.1, 1, 10, 100, and 1000 μM), and silica nanoparticles for 1, 2, and 3 days. RESULTS. Sodium nitrite and SNP showed a dose-dependent inhibitory effect on A. castellanii viability. A more prominent inhibitory effect was observed with SNP (less than 10{\%} of organisms survived at 7-day culture with 1000 μM) compared with sodium nitrite. However, more cytotoxicity on HCEC was observed with SNP. NO-releasing silica nanoparticles were successfully internalized into the amoebic cytoplasm and accumulated in large vacuoles. Although blank silica nanoparticles had no inhibitory effect on A. castellanii viability, NO-releasing silica nanoparticles showed a dose-dependent amoebicidal effect. Furthermore, no cystic transformation of A. castellanii was observed under a phase contrast microscope or transmission electron microscope after exogenous NO treatment. CONCLUSIONS. Our results demonstrated the anti-Acanthamoeba effect of exogenous NO. This finding suggests that NO-releasing drug platforms, including nano-carriers, can be a promising therapeutic strategy for Acanthamoeba keratitis.",
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AU - Jeong, Hyejoong

AU - Hong, Jinkee

AU - Kim, Martha

AU - Chang, Minwook

AU - Chuck, Roy S.

AU - Park, Choul Yong

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N2 - PURPOSE. Acanthamoeba keratitis is a well-known intractable corneal infectious disease. We investigated the anti-Acanthamoeba effect of exogenous nitric oxide (NO). METHODS. Acanthamoeba castellanii was axenically cultured and exposed to various concentrations of NO donors, such as sodium nitrite, sodium nitroprusside (SNP), and NO-releasing silica nanoparticles (coated in branched polyethylene imine, size:100 nm), for 1 to 7 days (sodium nitrite and SNP: 0, 0.1, 1, 10, 100, and 1000 μM; silica nanoparticles: 0, 6.25, 12.5, 25, 50, and 100 μg/mL). Human corneal epithelial cells (HCECs) were cultured and exposed to sodium nitrite, SNP (0, 0.1, 1, 10, 100, and 1000 μM), and silica nanoparticles for 1, 2, and 3 days. RESULTS. Sodium nitrite and SNP showed a dose-dependent inhibitory effect on A. castellanii viability. A more prominent inhibitory effect was observed with SNP (less than 10% of organisms survived at 7-day culture with 1000 μM) compared with sodium nitrite. However, more cytotoxicity on HCEC was observed with SNP. NO-releasing silica nanoparticles were successfully internalized into the amoebic cytoplasm and accumulated in large vacuoles. Although blank silica nanoparticles had no inhibitory effect on A. castellanii viability, NO-releasing silica nanoparticles showed a dose-dependent amoebicidal effect. Furthermore, no cystic transformation of A. castellanii was observed under a phase contrast microscope or transmission electron microscope after exogenous NO treatment. CONCLUSIONS. Our results demonstrated the anti-Acanthamoeba effect of exogenous NO. This finding suggests that NO-releasing drug platforms, including nano-carriers, can be a promising therapeutic strategy for Acanthamoeba keratitis.

AB - PURPOSE. Acanthamoeba keratitis is a well-known intractable corneal infectious disease. We investigated the anti-Acanthamoeba effect of exogenous nitric oxide (NO). METHODS. Acanthamoeba castellanii was axenically cultured and exposed to various concentrations of NO donors, such as sodium nitrite, sodium nitroprusside (SNP), and NO-releasing silica nanoparticles (coated in branched polyethylene imine, size:100 nm), for 1 to 7 days (sodium nitrite and SNP: 0, 0.1, 1, 10, 100, and 1000 μM; silica nanoparticles: 0, 6.25, 12.5, 25, 50, and 100 μg/mL). Human corneal epithelial cells (HCECs) were cultured and exposed to sodium nitrite, SNP (0, 0.1, 1, 10, 100, and 1000 μM), and silica nanoparticles for 1, 2, and 3 days. RESULTS. Sodium nitrite and SNP showed a dose-dependent inhibitory effect on A. castellanii viability. A more prominent inhibitory effect was observed with SNP (less than 10% of organisms survived at 7-day culture with 1000 μM) compared with sodium nitrite. However, more cytotoxicity on HCEC was observed with SNP. NO-releasing silica nanoparticles were successfully internalized into the amoebic cytoplasm and accumulated in large vacuoles. Although blank silica nanoparticles had no inhibitory effect on A. castellanii viability, NO-releasing silica nanoparticles showed a dose-dependent amoebicidal effect. Furthermore, no cystic transformation of A. castellanii was observed under a phase contrast microscope or transmission electron microscope after exogenous NO treatment. CONCLUSIONS. Our results demonstrated the anti-Acanthamoeba effect of exogenous NO. This finding suggests that NO-releasing drug platforms, including nano-carriers, can be a promising therapeutic strategy for Acanthamoeba keratitis.

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