Effect of microgravity on gene expression in mouse brain

Antonio Frigeri, Dumitru A. Iacobas, Sanda Iacobas, Grazia Paola Nicchia, Jean Francois Desaphy, Diana Conte Camerino, Maria Svelto, David C. Spray

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Changes in gravitational force such as that experienced by astronauts during space flight induce a redistribution of fluids from the caudad to the cephalad portion of the body together with an elimination of normal head-to-foot hydrostatic pressure gradients. To assess brain gene profile changes associated with microgravity and fluid shift, a large-scale analysis of mRNA expression levels was performed in the brains of 2-week control and hindlimb-unloaded (HU) mice using cDNA microarrays. Although to different extents, all functional categories displayed significantly regulated genes indicating that considerable transcriptomic alterations are induced by HU. Interestingly, the TIC class (transport of small molecules and ions into the cells) had the highest percentage of up-regulated genes, while the most down-regulated genes were those of the JAE class (cell junction, adhesion, extracellular matrix). TIC genes comprised 16% of those whose expression was altered, including sodium channel, nonvoltage-gated 1 beta (Scnn1b), glutamate receptor (Grin1), voltage-dependent anion channel 1 (Vdac1), calcium channel beta 3 subunit (Cacnb3) and others. The analysis performed by GeneMAPP revealed several altered protein classes and functional pathways such as blood coagulation and immune response, learning and memory, ion channels and cell junction. In particular, data indicate that HU causes an alteration in hemostasis which resolves in a shift toward a more hyper-coagulative state with an increased risk of venous thrombosis. Furthermore, HU treatment seems to impact on key steps of synaptic plasticity and learning processes.

Original languageEnglish (US)
Pages (from-to)289-300
Number of pages12
JournalExperimental Brain Research
Volume191
Issue number3
DOIs
StatePublished - Nov 2008

Fingerprint

Weightlessness
Hindlimb
Gene Expression
Brain
Intercellular Junctions
Genes
Voltage-Dependent Anion Channel 1
Fluid Shifts
Learning
Astronauts
Space Flight
Neuronal Plasticity
Hydrostatic Pressure
Sodium Channels
Glutamate Receptors
Blood Coagulation
Calcium Channels
Oligonucleotide Array Sequence Analysis
Hemostasis
Ion Channels

Keywords

  • Brain
  • cDNA microarray
  • Gravitational force
  • Hindlimb-unloaded
  • Microgravitational adaptations
  • Microgravity
  • Space flight

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Frigeri, A., Iacobas, D. A., Iacobas, S., Nicchia, G. P., Desaphy, J. F., Camerino, D. C., ... Spray, D. C. (2008). Effect of microgravity on gene expression in mouse brain. Experimental Brain Research, 191(3), 289-300. https://doi.org/10.1007/s00221-008-1523-5

Effect of microgravity on gene expression in mouse brain. / Frigeri, Antonio; Iacobas, Dumitru A.; Iacobas, Sanda; Nicchia, Grazia Paola; Desaphy, Jean Francois; Camerino, Diana Conte; Svelto, Maria; Spray, David C.

In: Experimental Brain Research, Vol. 191, No. 3, 11.2008, p. 289-300.

Research output: Contribution to journalArticle

Frigeri, A, Iacobas, DA, Iacobas, S, Nicchia, GP, Desaphy, JF, Camerino, DC, Svelto, M & Spray, DC 2008, 'Effect of microgravity on gene expression in mouse brain', Experimental Brain Research, vol. 191, no. 3, pp. 289-300. https://doi.org/10.1007/s00221-008-1523-5
Frigeri A, Iacobas DA, Iacobas S, Nicchia GP, Desaphy JF, Camerino DC et al. Effect of microgravity on gene expression in mouse brain. Experimental Brain Research. 2008 Nov;191(3):289-300. https://doi.org/10.1007/s00221-008-1523-5
Frigeri, Antonio ; Iacobas, Dumitru A. ; Iacobas, Sanda ; Nicchia, Grazia Paola ; Desaphy, Jean Francois ; Camerino, Diana Conte ; Svelto, Maria ; Spray, David C. / Effect of microgravity on gene expression in mouse brain. In: Experimental Brain Research. 2008 ; Vol. 191, No. 3. pp. 289-300.
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