Effect of methotrexate doxorubicin and mitoxantrone on human peritoneal mesothelial cell function in vitro

J. Witowski, A. Breborowicz, J. Knapowski

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Recent studies have demonstrated that intraperitoneal instillation of dialysis solutions and drug additives may adversely affect the function of peritoneal cell populations. Therefore the aim of the present investigation was to examine the short-term effects of antineoplastic agents on human peritoneal mesothelial cells (HPMC). We have assessed the integrity of HPMC membrane and mechanisms of intracellular potassium transport. There was no evidence of significant cytotoxicity (as measured by 86Rb release) during a 60-min exposure of HPMC to either methotrexate (10-6-10-4M), doxorubicin (10-7-10-5 M) or mitoxantrone (10-7-10-5 M). In HPMC exposed to doxorubicin (10-6 M) the intracellular transport of potassium, as assessed with 86Rb as its analogue, was not affected. Methotrexate (10-5 M) diminished Na,K-ATPase activity and simultaneously enhanced the 86Rb transport via furosemide-sensitive pathway. Mitoxantrone reduced the furosemide-sensitive 86Rb influx in a dose-dependent manner and at a concentration of 10-4 M it also impaired the ouabain-dependent 86Rb influx. These data demonstrate that antineoplastic agents interfere with HPMC function which might contribute to the oncostatic-induced peritoneal toxicity.

Original languageEnglish (US)
Pages (from-to)60-65
Number of pages6
JournalONCOLOGY (United States)
Volume52
Issue number1
StatePublished - 1995
Externally publishedYes

Fingerprint

Mitoxantrone
Methotrexate
Doxorubicin
Furosemide
Antineoplastic Agents
Potassium
Dialysis Solutions
Ouabain
Cell Membrane
In Vitro Techniques
Pharmaceutical Preparations
Population

Keywords

  • Cytotoxicity
  • Doxorubicin
  • Intraperitoneal chemotherapy
  • Mesothelial cells
  • Methotrexate
  • Mitoxantrone
  • Potassium transport

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Effect of methotrexate doxorubicin and mitoxantrone on human peritoneal mesothelial cell function in vitro. / Witowski, J.; Breborowicz, A.; Knapowski, J.

In: ONCOLOGY (United States), Vol. 52, No. 1, 1995, p. 60-65.

Research output: Contribution to journalArticle

Witowski, J. ; Breborowicz, A. ; Knapowski, J. / Effect of methotrexate doxorubicin and mitoxantrone on human peritoneal mesothelial cell function in vitro. In: ONCOLOGY (United States). 1995 ; Vol. 52, No. 1. pp. 60-65.
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