TY - JOUR
T1 - Effect of high pressure and reversed micelles on the fluorescent proteins
AU - Verkhusha, Vladislav V.
AU - Pozhitkov, Alexander E.
AU - Smirnov, Sergey A.
AU - Borst, Jan Willem
AU - Van Hoek, Arie
AU - Klyachko, Natalya L.
AU - Levashov, Andrey V.
AU - Visser, Antonie J.W.G.
N1 - Funding Information:
This work was supported in part by INTAS 2001-2347, NWO 047-007-005 and LST.CLG 974984 grants.
PY - 2003/8/22
Y1 - 2003/8/22
N2 - Two physico-chemical perturbations were applied to ECFP, EGFP, EYFP and DsRed fluorescent proteins: high hydrostatic pressure and encapsulation in reversed micelles. The observed fluorescence changes were described by two-state model and quantified by thermodynamic formalism. ECFP, EYFP and DsRed exhibited similar reaction volumes under pressure. The changes of the chemical potentials of the chromophore in bis(2-ethylhexyl)sulfosuccinate (AOT) micelles caused apparent chromophore protonation changes resulting in a fluorescence decrease of ECFP and EYFP. In contrast to the remarkable stability of DsRed, the highest sensitivity of EYFP fluorescence under pressure and in micelles is attributed to its chromophore structure.
AB - Two physico-chemical perturbations were applied to ECFP, EGFP, EYFP and DsRed fluorescent proteins: high hydrostatic pressure and encapsulation in reversed micelles. The observed fluorescence changes were described by two-state model and quantified by thermodynamic formalism. ECFP, EYFP and DsRed exhibited similar reaction volumes under pressure. The changes of the chemical potentials of the chromophore in bis(2-ethylhexyl)sulfosuccinate (AOT) micelles caused apparent chromophore protonation changes resulting in a fluorescence decrease of ECFP and EYFP. In contrast to the remarkable stability of DsRed, the highest sensitivity of EYFP fluorescence under pressure and in micelles is attributed to its chromophore structure.
KW - Chemical potential
KW - Chromophore protonation
KW - Reaction volume
KW - Thermodynamics
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U2 - 10.1016/S0304-4165(03)00140-5
DO - 10.1016/S0304-4165(03)00140-5
M3 - Article
C2 - 12928115
AN - SCOPUS:0042591403
SN - 0304-4165
VL - 1622
SP - 192
EP - 195
JO - Biochimica et Biophysica Acta - General Subjects
JF - Biochimica et Biophysica Acta - General Subjects
IS - 3
ER -