Effect of high pressure and reversed micelles on the fluorescent proteins

Vladislav V. Verkhusha, Alexander E. Pozhitkov, Sergey A. Smirnov, Jan Willem Borst, Arie Van Hoek, Natalya L. Klyachko, Andrey V. Levashov, Antonie J.W.G. Visser

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Two physico-chemical perturbations were applied to ECFP, EGFP, EYFP and DsRed fluorescent proteins: high hydrostatic pressure and encapsulation in reversed micelles. The observed fluorescence changes were described by two-state model and quantified by thermodynamic formalism. ECFP, EYFP and DsRed exhibited similar reaction volumes under pressure. The changes of the chemical potentials of the chromophore in bis(2-ethylhexyl)sulfosuccinate (AOT) micelles caused apparent chromophore protonation changes resulting in a fluorescence decrease of ECFP and EYFP. In contrast to the remarkable stability of DsRed, the highest sensitivity of EYFP fluorescence under pressure and in micelles is attributed to its chromophore structure.

Original languageEnglish (US)
Pages (from-to)192-195
Number of pages4
JournalBiochimica et Biophysica Acta - General Subjects
Volume1622
Issue number3
DOIs
StatePublished - Aug 22 2003
Externally publishedYes

Keywords

  • Chemical potential
  • Chromophore protonation
  • Reaction volume
  • Thermodynamics

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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  • Cite this

    Verkhusha, V. V., Pozhitkov, A. E., Smirnov, S. A., Borst, J. W., Van Hoek, A., Klyachko, N. L., Levashov, A. V., & Visser, A. J. W. G. (2003). Effect of high pressure and reversed micelles on the fluorescent proteins. Biochimica et Biophysica Acta - General Subjects, 1622(3), 192-195. https://doi.org/10.1016/S0304-4165(03)00140-5