Early response to tendon fatigue damage accumulation in a novel in vivo model

David T. Fung, Vincent M. Wang, Nelly Andarawis-Puri, Jelena Basta-Pljakic, Yonghui Li, Damien M. Laudier, Hui (Herb) Sun, Karl J. Jepsen, Mitchell B. Schaffler, Evan L. Flatow

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

This study describes the development and application of a novel rat patellar tendon model of mechanical fatigue for investigating the early in vivo response to tendon subfailure injury. Patellar tendons of adult female Sprague-Dawley rats were fatigue loaded between 1-35 N using a custom-designed loading apparatus. Patellar tendons were subjected to Low-, Moderate- or High-level fatigue damage, defined by grip-to-grip strain measurement. Molecular response was compared with that of a laceration-repair injury. Histological analyses showed that progression of tendon fatigue involves formation of localized kinked fiber deformations at Low damage, which increased in density with presence of fiber delaminations at Moderate damage, and fiber angulation and discontinuities at High damage levels. RT-PCR analysis performed at 1- and 3-day post-fatigue showed variable changes in type I, III and V collagen mRNA expression at Low and Moderate damage levels, consistent with clinical findings of tendon pathology and were modest compared with those observed at High damage levels, in which expression of all collagens evaluated were increased markedly. In contrast, only type I collagen expression was elevated at the same time points post-laceration. Findings suggest that cumulative fatigue in tendon invokes a different molecular response than laceration. Further, structural repair may not be initiated until reaching end-stage fatigue life, where the repair response may unable to restore the damaged tendon to its pre-fatigue architecture.

Original languageEnglish (US)
Pages (from-to)274-279
Number of pages6
JournalJournal of Biomechanics
Volume43
Issue number2
DOIs
StatePublished - Jan 19 2010
Externally publishedYes

Fingerprint

Tendons
Fatigue damage
Fatigue
Fatigue of materials
Patellar Ligament
Lacerations
Collagen
Repair
Hand Strength
Collagen Type I
Fibers
Rats
Collagen Type V
Tendon Injuries
Collagen Type III
Strain measurement
Pathology
Delamination
Sprague Dawley Rats
Polymerase Chain Reaction

Keywords

  • Animal model
  • Biomechanics
  • Damage accumulation
  • Fatigue
  • Histology
  • Imaging
  • Morphology
  • Second harmonic generation
  • Tendinopathy
  • Tendon
  • Tendon injury

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Rehabilitation
  • Biophysics
  • Biomedical Engineering

Cite this

Fung, D. T., Wang, V. M., Andarawis-Puri, N., Basta-Pljakic, J., Li, Y., Laudier, D. M., ... Flatow, E. L. (2010). Early response to tendon fatigue damage accumulation in a novel in vivo model. Journal of Biomechanics, 43(2), 274-279. https://doi.org/10.1016/j.jbiomech.2009.08.039

Early response to tendon fatigue damage accumulation in a novel in vivo model. / Fung, David T.; Wang, Vincent M.; Andarawis-Puri, Nelly; Basta-Pljakic, Jelena; Li, Yonghui; Laudier, Damien M.; Sun, Hui (Herb); Jepsen, Karl J.; Schaffler, Mitchell B.; Flatow, Evan L.

In: Journal of Biomechanics, Vol. 43, No. 2, 19.01.2010, p. 274-279.

Research output: Contribution to journalArticle

Fung, DT, Wang, VM, Andarawis-Puri, N, Basta-Pljakic, J, Li, Y, Laudier, DM, Sun, HH, Jepsen, KJ, Schaffler, MB & Flatow, EL 2010, 'Early response to tendon fatigue damage accumulation in a novel in vivo model', Journal of Biomechanics, vol. 43, no. 2, pp. 274-279. https://doi.org/10.1016/j.jbiomech.2009.08.039
Fung DT, Wang VM, Andarawis-Puri N, Basta-Pljakic J, Li Y, Laudier DM et al. Early response to tendon fatigue damage accumulation in a novel in vivo model. Journal of Biomechanics. 2010 Jan 19;43(2):274-279. https://doi.org/10.1016/j.jbiomech.2009.08.039
Fung, David T. ; Wang, Vincent M. ; Andarawis-Puri, Nelly ; Basta-Pljakic, Jelena ; Li, Yonghui ; Laudier, Damien M. ; Sun, Hui (Herb) ; Jepsen, Karl J. ; Schaffler, Mitchell B. ; Flatow, Evan L. / Early response to tendon fatigue damage accumulation in a novel in vivo model. In: Journal of Biomechanics. 2010 ; Vol. 43, No. 2. pp. 274-279.
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