Early pulmonary cytokine and chemokine responses in mice immunized with three different vaccines against Mycobacterium tuberculosis determined by PCR array

JaeHyun Lim, Steven C. Derrick, Kristopher Kolibab, Amy Li Yang, Steven A. Porcelli, William R. Jacobs, Sheldon L. Morris

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

In this study, the early pulmonary cytokine and chemokine responses in mice immunized with either BCG vaccine, a ΔsecA2 mutant of Mycobacterium tuberculosis, or a DNA vaccine expressing an ESAT6-antigen 85B fusion protein and then aerogenically challenged with a low dose of M. tuberculosis were evaluated by PCR array. The cellular immune responses at day 10 postchallenge were essentially equivalent in the lungs of mice immunized with either the highly immunogenic BCG vaccine or the ΔsecA2 M. tuberculosis mutant strain. Specifically, 12 immune biomolecules (including gamma interferon [IFN-γ], interleukin-21 [IL-21], IL-27, IL-17f, CXCL9, CXCL10, and CXCL11) were differentially regulated, relative to the levels for naïve controls, in the lungs of vaccinated mice at this time point. Although the vaccine-related immune responses evoked in mice immunized with the DNA vaccine were relatively limited at 10 days postinfection, upregulation of IFN-γ RNA synthesis as well as increased expression levels of CXCL9, CXCL10, and CXCL11 chemokines were detected.

Original languageEnglish (US)
Pages (from-to)122-126
Number of pages5
JournalClinical and Vaccine Immunology
Volume16
Issue number1
DOIs
StatePublished - Jan 2009

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BCG Vaccine
DNA Vaccines
Mycobacterium tuberculosis
Chemokines
Chemokine CXCL11
Chemokine CXCL9
Vaccines
Interleukin-27
Chemokine CXCL10
Cytokines
Polymerase Chain Reaction
Lung
Interleukin-17
Biomolecules
Interferons
Interferon-gamma
Fusion reactions
RNA
Antigens
Cellular Immunity

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Immunology
  • Immunology and Allergy
  • Microbiology (medical)

Cite this

Early pulmonary cytokine and chemokine responses in mice immunized with three different vaccines against Mycobacterium tuberculosis determined by PCR array. / Lim, JaeHyun; Derrick, Steven C.; Kolibab, Kristopher; Yang, Amy Li; Porcelli, Steven A.; Jacobs, William R.; Morris, Sheldon L.

In: Clinical and Vaccine Immunology, Vol. 16, No. 1, 01.2009, p. 122-126.

Research output: Contribution to journalArticle

Lim, JaeHyun ; Derrick, Steven C. ; Kolibab, Kristopher ; Yang, Amy Li ; Porcelli, Steven A. ; Jacobs, William R. ; Morris, Sheldon L. / Early pulmonary cytokine and chemokine responses in mice immunized with three different vaccines against Mycobacterium tuberculosis determined by PCR array. In: Clinical and Vaccine Immunology. 2009 ; Vol. 16, No. 1. pp. 122-126.
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