Early cell transplantation in LEC rats modeling Wilson's disease eliminates hepatic copper with reversal of liver disease

Harmeet Malhi, Adil N. Irani, Sanjeev Gupta, Irene Volenberg, Michael L. Schilsky

Research output: Contribution to journalArticlepeer-review

61 Scopus citations

Abstract

Background & Aims: The Long-Evans Cinnamon (LEC) rat is an excellent model of Wilson's disease with impaired copper excretion, hypoceruloplasminemia, and copper toxicosis. We hypothesized that early hepatocyte transplantation would improve copper excretion and liver disease in Wilson's disease. Methods: Normal syngeneic Long-Evans Agouti rat hepatocytes were transplanted intrasplenically into 2-week-old LEC rats. Untreated LEC pups were controls. Liver repopulation was shown by changes in serum ceruloplasmin, hepatic atp7b messenger RNA, and bile and liver copper levels. Histologic analysis of the liver was performed. Results: Significant copper accumulation and liver disease were observed in 5-month-old LEC rats, with occasional treated rats showing increased bile copper excretion. The liver was repopulated extensively in 10 of 14 treated LEC rats (71%) 20 months after cell transplantation. In these 10 rats, hepatic copper content was virtually normal in 6 rats (53 ± 12 μg/g liver) and substantially less in 4 others (270 ± 35 μg/g) compared with elevated liver copper levels in untreated LEC rats (1090 ± 253 μg/g) (P < 0.001). Changes in serum ceruloplasmin levels, bile copper excretion capacity, and liver histology were in concordance with decreases in liver copper levels. Conclusions: Transplanted cells proliferated subsequent to the onset of liver injury, and the liver was repopulated over an extended period. Cell transplantation eventually restored copper homeostasis and reversed liver disease without hepatic preconditioning in LEC rats.

Original languageEnglish (US)
Pages (from-to)438-447
Number of pages10
JournalGastroenterology
Volume122
Issue number2
DOIs
StatePublished - 2002

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

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