Dynamics of the rho-family small GTPases in actin regulation and motility

Désirée Spiering, Louis Hodgson

Research output: Contribution to journalReview article

172 Scopus citations

Abstract

The p21 Rho-family of small GTP ases are master regulators of actin cytoskeleton rearrangements. Their functions have been well characterized in terms of their effects toward various actin-modulating protein targets. However, more recent studies have shown that the dynamics of Rho GTP ase activities are highly complex and tightly regulated in order to achieve their specific subcellular localization. Furthermore, these localized effects are highly dynamic, often spanning the time-scale of seconds, making the interpretation of traditional biochemical approaches inadequate to fully decipher these rapid mechanisms in vivo. Here, we provide an overview of Rho family GTP ase biology, and introduce state-of-the-art approaches to study the dynamics of these important signaling proteins that ultimately coordinate the actin cytoskeleton rearrangements during cell migration.

Original languageEnglish (US)
Pages (from-to)170-180
Number of pages11
JournalCell Adhesion and Migration
Volume5
Issue number2
DOIs
StatePublished - Jan 1 2011

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Keywords

  • Actin
  • Biosensors
  • Dynamics
  • FRET
  • Live-cell imaging
  • Migration
  • Rho

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Cell Biology

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