Dynamics of microtubules, motor proteins and 20S proteasomes during bovine oocyte IVM

Silvia Racedo, M. C. Branzini, D. Salamone, C. Wójcik, V. Y. Rawe, H. Niemann

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The present study investigated the distribution of cytoplasmic dynein, dynactin and 20S proteasomes in oocytes isolated from small (<2 mm) and large (2-8 mm) follicles during IVM. Immediately after chromatin condensation (germinal vesicle (GV) breakdown), dynactin was closely associated with the chromatin and interacted with tubulin at the MI and MII spindles in oocytes recovered from large follicles. Dynactin showed perinuclear concentration. Dynein was homogeneously distributed in the cytoplasm of GV oocytes in both groups and was associated with the chromatin at the MI and MII spindle. The 20S proteasomes were found predominantly in the nucleus at the GV stage and were associated with the chromatin up to the MII stage in both groups of oocytes. The use of sodium orthovanadate, an inhibitor or phosphatase and ATPase activity, and nocodazole, a known disruptor of microtubules, affected the localisation of proteasomes in the meiotic stages. The results demonstrate the distinct dynamics of molecular motors and proteasomes during bovine oocyte IVM, their possible relationship with the developmental competence of the oocyte and the link between microtubules, their associated molecular motors and the transport of proteasomes during bovine female meiosis.

Original languageEnglish (US)
Pages (from-to)304-312
Number of pages9
JournalReproduction, Fertility and Development
Volume21
Issue number2
DOIs
StatePublished - 2009
Externally publishedYes

Fingerprint

Microtubule Proteins
proteasome endopeptidase complex
Proteasome Endopeptidase Complex
microtubules
Oocytes
oocytes
germinal vesicle
Chromatin
chromatin
cattle
proteins
Microtubules
Cytoplasmic Dyneins
Nocodazole
Dyneins
Vanadates
molecular dynamics
Meiosis
Molecular Dynamics Simulation
Tubulin

Keywords

  • Developmental competence
  • Follicle size
  • Molecular motors

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Genetics
  • Endocrinology
  • Developmental Biology
  • Reproductive Medicine
  • Animal Science and Zoology

Cite this

Dynamics of microtubules, motor proteins and 20S proteasomes during bovine oocyte IVM. / Racedo, Silvia; Branzini, M. C.; Salamone, D.; Wójcik, C.; Rawe, V. Y.; Niemann, H.

In: Reproduction, Fertility and Development, Vol. 21, No. 2, 2009, p. 304-312.

Research output: Contribution to journalArticle

Racedo, Silvia ; Branzini, M. C. ; Salamone, D. ; Wójcik, C. ; Rawe, V. Y. ; Niemann, H. / Dynamics of microtubules, motor proteins and 20S proteasomes during bovine oocyte IVM. In: Reproduction, Fertility and Development. 2009 ; Vol. 21, No. 2. pp. 304-312.
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