Dynamic metabolic transformation in tumor invasion and metastasis in mice with LM-8 osteosarcoma cell transplantation

Yingqi Hua, Yunping Qiu, Aihua Zhao, Xiaoyan Wang, Tianlu Chen, Zhiyu Zhang, Yi Chi, Quan Li, Wei Sun, Guodong Li, Zhengdong Cai, Zhanxiang Zhou, Wei Jia

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

While extensive evidence indicates that tumor cells shift their global metabolic programs, the molecular details of the metabolic transformation in tumor invasion, progression, and metastasis remain largely unknown. Characterization of the time-dependent metabolic shift during the tumor invasion, development, and metastasis will describe an important aspect of tumor phenotypes and potentially allow us to design therapies that inhibit tumor cell movement. In this study, a metabonomic study was performed to characterize the global metabolic changes during the process of tumor invasion and metastasis to lung in a mouse model with subcutaneous transplantation of murine osteosarcoma cell line (LM8). The serum metabolic profiling revealed that many key metabolites in glycolysis and tricarboxylic acid (TCA) cycle, as well as most of the amino acids were elevated at rapidly growing stage of tumor, presumably resulting from a high energy demand and turnover of anabolic metabolism during the tumor cell proliferation. Serum levels of succinic acid and proline significantly increased (with fold change FC = 10.75 and 4.43, relative to controls) among all the metabolites in the third week. The serum metabolic profile of lung metastasis at week 4 was different from that at week 3, in that most of previously increased serum metabolites were found decreased, except for cholesterol and several free fatty acids, suggesting lowered carbohydrate and amino acids metabolism, but an elevated lipid metabolism associated with tumor metastasis.

Original languageEnglish (US)
Pages (from-to)3513-3521
Number of pages9
JournalJournal of Proteome Research
Volume10
Issue number8
DOIs
StatePublished - Aug 5 2011
Externally publishedYes

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Cell Transplantation
Osteosarcoma
Tumors
Neoplasm Metastasis
Neoplasms
Metabolites
Cells
Serum
Metabolism
Amino Acids
Lung
Metabolomics
Citric Acid Cycle
Metabolome
Cell proliferation
Succinic Acid
Glycolysis
Lipid Metabolism
Nonesterified Fatty Acids
Proline

Keywords

  • invasion
  • metabolic transformation
  • metabolomics
  • metabonomics
  • metastasis
  • osteosarcoma

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

Dynamic metabolic transformation in tumor invasion and metastasis in mice with LM-8 osteosarcoma cell transplantation. / Hua, Yingqi; Qiu, Yunping; Zhao, Aihua; Wang, Xiaoyan; Chen, Tianlu; Zhang, Zhiyu; Chi, Yi; Li, Quan; Sun, Wei; Li, Guodong; Cai, Zhengdong; Zhou, Zhanxiang; Jia, Wei.

In: Journal of Proteome Research, Vol. 10, No. 8, 05.08.2011, p. 3513-3521.

Research output: Contribution to journalArticle

Hua, Y, Qiu, Y, Zhao, A, Wang, X, Chen, T, Zhang, Z, Chi, Y, Li, Q, Sun, W, Li, G, Cai, Z, Zhou, Z & Jia, W 2011, 'Dynamic metabolic transformation in tumor invasion and metastasis in mice with LM-8 osteosarcoma cell transplantation', Journal of Proteome Research, vol. 10, no. 8, pp. 3513-3521. https://doi.org/10.1021/pr200147g
Hua, Yingqi ; Qiu, Yunping ; Zhao, Aihua ; Wang, Xiaoyan ; Chen, Tianlu ; Zhang, Zhiyu ; Chi, Yi ; Li, Quan ; Sun, Wei ; Li, Guodong ; Cai, Zhengdong ; Zhou, Zhanxiang ; Jia, Wei. / Dynamic metabolic transformation in tumor invasion and metastasis in mice with LM-8 osteosarcoma cell transplantation. In: Journal of Proteome Research. 2011 ; Vol. 10, No. 8. pp. 3513-3521.
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