Dynamic control of hERG/IKr by PKA-mediated interactions with 14-3-3

Anna Kagan, Thomas V. McDonald

Research output: Chapter in Book/Report/Conference proceedingConference contribution

12 Scopus citations

Abstract

IKs has been considered the potassium current most responsible for adrenergic/cAMP-mediated changes in cardiac repolarization during stress. Increasing biochemical, electrophysiological and genetic evidence however, points to a role for hERG/IKr in β-adrenergic responses. Elevations of cAMP as seen in β-adrenergic stimulation can result in PKA-dependent phosphorylation of hERG and direct binding of cAMP to the channel protein. Generally, there is a suppression of current density due to the channel phosphorylation. We recently identified a novel protein-protein interaction between hERG and the adaptor protein 14-3-3ε. Interaction sites exist on both N- and C-termini of hERG and the interaction is dynamic, requiring phosphorylation of the channel by PKA. When both sites bind to 14-3-3 proteins there is an acceleration and augmentation of current activation in contrast to the depression of current with phosphorylation alone. When sufficient 14-3-3 is available the phosphorylation state of the channel is stabilized and prolonged. Thus, 14-3-3 interactions with hERG provide a unique mechanism for plasticity in the autonomic control of stress-dependent regulation of cardiac membrane excitability. Here, we summarize our findings and report on our further efforts to analyse interactions between the native channel protein and 14-3-3 in cardiac myocytes.

Original languageEnglish (US)
Title of host publicationThe hERG Cardiac Potassium Channel
Subtitle of host publicationStructure, Function and Long QT Syndrome
Pages75-89
Number of pages15
StatePublished - 2005

Publication series

NameNovartis Foundation Symposium
Volume266
ISSN (Print)1528-2511

ASJC Scopus subject areas

  • Medicine(all)

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