Dynamic assembly of brambleberry mediates nuclear envelope fusion during early development

Elliott W. Abrams, Hong Zhang, Florence L. Marlow, Lee Kapp, Sumei Lu, Mary C. Mullins

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

To accommodate the large cells following zygote formation, early blastomeres employ modified cell divisions. Karyomeres are one such modification, mitotic intermediates wherein individual chromatin masses are surrounded by nuclear envelope; the karyomeres then fuse to form a single mononucleus. We identified brambleberry, a maternal-effect zebrafish mutant that disrupts karyomere fusion, resulting in formation of multiple micronuclei. As karyomeres form, Brambleberry protein localizes to the nuclear envelope, with prominent puncta evident near karyomere-karyomere interfaces corresponding to membrane fusion sites. brambleberry corresponds to an unannotated gene with similarity to Kar5p, a protein that participates in nuclear fusion in yeast. We also demonstrate that Brambleberry is required for pronuclear fusion following fertilization in zebrafish. Our studies provide insight into the machinery required for karyomere fusion and suggest that specialized proteins are necessary for proper nuclear division in large dividing blastomeres.

Original languageEnglish (US)
Pages (from-to)521-532
Number of pages12
JournalCell
Volume150
Issue number3
DOIs
StatePublished - Aug 3 2012
Externally publishedYes

Fingerprint

Nuclear Fusion
Nuclear Envelope
Blastomeres
Fusion reactions
Zebrafish
Cell Nucleus Division
Proteins
Membrane Fusion
Zygote
Fertilization
Cell Division
Chromatin
Yeasts
Electric fuses
Yeast
Machinery
Genes
Cells
Membranes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Abrams, E. W., Zhang, H., Marlow, F. L., Kapp, L., Lu, S., & Mullins, M. C. (2012). Dynamic assembly of brambleberry mediates nuclear envelope fusion during early development. Cell, 150(3), 521-532. https://doi.org/10.1016/j.cell.2012.05.048

Dynamic assembly of brambleberry mediates nuclear envelope fusion during early development. / Abrams, Elliott W.; Zhang, Hong; Marlow, Florence L.; Kapp, Lee; Lu, Sumei; Mullins, Mary C.

In: Cell, Vol. 150, No. 3, 03.08.2012, p. 521-532.

Research output: Contribution to journalArticle

Abrams, EW, Zhang, H, Marlow, FL, Kapp, L, Lu, S & Mullins, MC 2012, 'Dynamic assembly of brambleberry mediates nuclear envelope fusion during early development', Cell, vol. 150, no. 3, pp. 521-532. https://doi.org/10.1016/j.cell.2012.05.048
Abrams, Elliott W. ; Zhang, Hong ; Marlow, Florence L. ; Kapp, Lee ; Lu, Sumei ; Mullins, Mary C. / Dynamic assembly of brambleberry mediates nuclear envelope fusion during early development. In: Cell. 2012 ; Vol. 150, No. 3. pp. 521-532.
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