Drosophila RpS12 controls translation, growth, and cell competition through Xrp1

Zhejun Ji, Marianthi Kiparaki, Virginia Folgado, Amit Kumar, Jorge Blanco, Gerard Rimesso, Jacky Chuen, Yang Liu, Deyou Zheng, Nicholas E. Baker

Research output: Contribution to journalArticle

Abstract

Whereas complete loss of Rp function is generally lethal, most heterozygous Rp mutants grow more slowly and are subject to competitive loss from mosaics tissues that also contain wild type cells. The rpS12 gene has a special role in the cell competition of other Ribosomal Protein (Rp) mutant cells in Drosophila. Elimination by cell competition is promoted by higher RpS12 levels and prevented by a specific rpS12 mis-sense mutation, identifying RpS12 as a key effector of cell competition due to mutations in other Rp genes. Here we show that RpS12 is also required for other aspects of Rp mutant phenotypes, including hundreds of gene expression changes that occur in ‘Minute’ Rp heterozygous wing imaginal discs, overall translation rate, and the overall rate of organismal development, all through the bZip protein Xrp1 that is one of the RpS12-regulated genes. Our findings outline the regulatory response to mutations affecting essential Rp genes that controls overall translation, growth, and cell competition, and which may contribute to cancer and other diseases.

Original languageEnglish (US)
Article numbere1008513
JournalPLoS genetics
Volume15
Issue number12
DOIs
StatePublished - Jan 1 2019

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Ribosomal Proteins
ribosomal proteins
translation (genetics)
Drosophila
protein
Growth
cells
mutation
gene
mutants
Mutation
Genes
genes
Imaginal Discs
imaginal discs
missense mutation
lethal genes
gene expression
phenotype
cancer

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Genetics(clinical)
  • Cancer Research

Cite this

Drosophila RpS12 controls translation, growth, and cell competition through Xrp1. / Ji, Zhejun; Kiparaki, Marianthi; Folgado, Virginia; Kumar, Amit; Blanco, Jorge; Rimesso, Gerard; Chuen, Jacky; Liu, Yang; Zheng, Deyou; Baker, Nicholas E.

In: PLoS genetics, Vol. 15, No. 12, e1008513, 01.01.2019.

Research output: Contribution to journalArticle

Ji, Z, Kiparaki, M, Folgado, V, Kumar, A, Blanco, J, Rimesso, G, Chuen, J, Liu, Y, Zheng, D & Baker, NE 2019, 'Drosophila RpS12 controls translation, growth, and cell competition through Xrp1', PLoS genetics, vol. 15, no. 12, e1008513. https://doi.org/10.1371/journal.pgen.1008513
Ji Z, Kiparaki M, Folgado V, Kumar A, Blanco J, Rimesso G et al. Drosophila RpS12 controls translation, growth, and cell competition through Xrp1. PLoS genetics. 2019 Jan 1;15(12). e1008513. https://doi.org/10.1371/journal.pgen.1008513
Ji, Zhejun ; Kiparaki, Marianthi ; Folgado, Virginia ; Kumar, Amit ; Blanco, Jorge ; Rimesso, Gerard ; Chuen, Jacky ; Liu, Yang ; Zheng, Deyou ; Baker, Nicholas E. / Drosophila RpS12 controls translation, growth, and cell competition through Xrp1. In: PLoS genetics. 2019 ; Vol. 15, No. 12.
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