Drosophila H1 regulates the genetic activity of heterochromatin by recruitment of Su(var)3-9

Xingwu Lu, Sandeep N. Wontakal, Harsh Kavi, Byung Ju Kim, Paloma M. Guzzardo, Alexander Emelyanov, Na Xu, Gregory J. Hannon, Jiri Zavadil, Dmitry Fyodorov, Arthur I. Skoultchi

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Eukaryotic genomes harbor transposable elements and other repetitive sequences that must be silenced. Small RNA interference pathways play a major role in their repression. Here, we reveal another mechanism for silencing these sequences in Drosophila. Depleting the linker histone H1 in vivo leads to strong activation of these elements. H1-mediated silencing occurs in combination with the heterochromatin-specific histone H3 lysine 9 methyltransferase Su(var)3-9. H1 physically interacts with Su(var)3-9 and recruits it to chromatin in vitro, which promotes H3 methylation. We propose that H1 plays a key role in silencing by tethering Su(var)3-9 to heterochromatin. The tethering function of H1 adds to its established role as a regulator of chromatin compaction and accessibility.

Original languageEnglish (US)
Pages (from-to)78-81
Number of pages4
JournalScience
Volume340
Issue number6128
DOIs
StatePublished - Apr 5 2013

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Personnel Selection
Heterochromatin
Histones
Drosophila
Chromatin
DNA Transposable Elements
Nucleic Acid Repetitive Sequences
Methyltransferases
RNA Interference
Methylation
Lysine
Genome
In Vitro Techniques

ASJC Scopus subject areas

  • General
  • Medicine(all)

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Drosophila H1 regulates the genetic activity of heterochromatin by recruitment of Su(var)3-9. / Lu, Xingwu; Wontakal, Sandeep N.; Kavi, Harsh; Kim, Byung Ju; Guzzardo, Paloma M.; Emelyanov, Alexander; Xu, Na; Hannon, Gregory J.; Zavadil, Jiri; Fyodorov, Dmitry; Skoultchi, Arthur I.

In: Science, Vol. 340, No. 6128, 05.04.2013, p. 78-81.

Research output: Contribution to journalArticle

Lu, X, Wontakal, SN, Kavi, H, Kim, BJ, Guzzardo, PM, Emelyanov, A, Xu, N, Hannon, GJ, Zavadil, J, Fyodorov, D & Skoultchi, AI 2013, 'Drosophila H1 regulates the genetic activity of heterochromatin by recruitment of Su(var)3-9', Science, vol. 340, no. 6128, pp. 78-81. https://doi.org/10.1126/science.1234654
Lu, Xingwu ; Wontakal, Sandeep N. ; Kavi, Harsh ; Kim, Byung Ju ; Guzzardo, Paloma M. ; Emelyanov, Alexander ; Xu, Na ; Hannon, Gregory J. ; Zavadil, Jiri ; Fyodorov, Dmitry ; Skoultchi, Arthur I. / Drosophila H1 regulates the genetic activity of heterochromatin by recruitment of Su(var)3-9. In: Science. 2013 ; Vol. 340, No. 6128. pp. 78-81.
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AU - Guzzardo, Paloma M.

AU - Emelyanov, Alexander

AU - Xu, Na

AU - Hannon, Gregory J.

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AU - Fyodorov, Dmitry

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