Down-regulation of types I, II and III inositol 1,4,5-trisphosphate receptors is mediated by the ubiquitin/proteasome pathway

Jon Oberdorf, Jack M. Webster, Changcheng Zhu, Su Ge Luo, Richard J H Wojcikiewicz

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Activation of certain phosphoinositidase-C-linked cell-surface receptors is known to cause an acceleration of the proteolysis of inositol 1,4,5-trisphosphate [Ins(1,4,5)P3] receptors and, thus, lead to Ins(1,4,5)P3-receptor down-regulation. In the current study we have sought to determine whether the ubiquitin/proteasome pathway is involved in this adaptive response. The data presented show (i) that activation of phosphoinositidase-C-linked receptors causes Ins(1,4,5)P3-receptor ubiquitination in a range of cell types (AR4-2J cells, INS-1 cells and rat cerebellar granule cells), (ii) that the Ins(1,4,5)P3-receptor down-regulation induced by activation of these receptors is blocked by proteasome inhibitors, (iii) that all known Ins(1,4,5)P3 receptors (types I, II and III) are substrates for ubiquitination, (iv) that ubiquitination occurs while Ins(1,4,5)P3 receptors are membrane-bound, (v) that Ins(1,4,5)P3-receptor ubiquitination and down-regulation are stimulated only by those agonists that elevate Ins(1,4,5)P3 concentration persistently, and (vi) that a portion of cellular Ins(1,4,5)P3 receptors (those that are not type-I-receptor-associated) can be resistant to ubiquitination and degradation. In total these data indicate that the ubiquitin/proteasome pathway mediates Ins(1,4,5)P3-receptor down-regulation and suggest that ubiquitination is stimulated by the binding of Ins(1,4,5)P3 to its receptor.

Original languageEnglish (US)
Pages (from-to)453-461
Number of pages9
JournalBiochemical Journal
Volume339
Issue number2
DOIs
StatePublished - Apr 15 1999
Externally publishedYes

Fingerprint

Inositol 1,4,5-Trisphosphate Receptors
Ubiquitination
Proteasome Endopeptidase Complex
Ubiquitin
Down-Regulation
Chemical activation
Proteolysis
Proteasome Inhibitors
Inositol 1,4,5-Trisphosphate
Cell Surface Receptors
Rats
Membranes
Degradation
Substrates
glycerophosphoinositol glycerophosphodiesterase

Keywords

  • Adaptation
  • Cell lines
  • Degradation

ASJC Scopus subject areas

  • Biochemistry

Cite this

Down-regulation of types I, II and III inositol 1,4,5-trisphosphate receptors is mediated by the ubiquitin/proteasome pathway. / Oberdorf, Jon; Webster, Jack M.; Zhu, Changcheng; Luo, Su Ge; Wojcikiewicz, Richard J H.

In: Biochemical Journal, Vol. 339, No. 2, 15.04.1999, p. 453-461.

Research output: Contribution to journalArticle

Oberdorf, Jon ; Webster, Jack M. ; Zhu, Changcheng ; Luo, Su Ge ; Wojcikiewicz, Richard J H. / Down-regulation of types I, II and III inositol 1,4,5-trisphosphate receptors is mediated by the ubiquitin/proteasome pathway. In: Biochemical Journal. 1999 ; Vol. 339, No. 2. pp. 453-461.
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