TY - JOUR
T1 - Dopamine antagonist haloperidol increases carboxypeptidase E mRNA in rat neurointermediate pituitary but not in various other rat tissues
AU - Grigoriants, Olga
AU - Devi, Lakshmi
AU - Fricker, Lloyd D.
N1 - Funding Information:
This work was supported by NIDA Grant DA-04494 and an Irma T. Hirschl Career Scientist Award (to LD.F.) and by NIH Grant NS-26880 (L.D.).
PY - 1993/7
Y1 - 1993/7
N2 - Carboxypeptidase E (CPE) is involved with the biosynthesis of many neuropeptides, including several whose genes are regulated by haloperidol treatments. In this study, we examined whether haloperidol alters CPE mRNA levels in a variety of tissues. Rats were treated for either 1, 3, 7, 14, or 21 days with 2 mg/kg haloperidol, and then Northern blot analysis performed on RNA from neurointermediate pituitary, anterior pituitary, hypothalamus, striatum, cerebellum, and adrenal. The 14 and 21 day treatments produced a significant 90-110% elevation of CPE mRNA in neurointermediate pituitary. However, the levels of CPE mRNA in the other tissues were not significantly influenced by the haloperidol treatments. This finding indicates that CPE is not co-regulated with peptide hormone mRNAs in all tissues.
AB - Carboxypeptidase E (CPE) is involved with the biosynthesis of many neuropeptides, including several whose genes are regulated by haloperidol treatments. In this study, we examined whether haloperidol alters CPE mRNA levels in a variety of tissues. Rats were treated for either 1, 3, 7, 14, or 21 days with 2 mg/kg haloperidol, and then Northern blot analysis performed on RNA from neurointermediate pituitary, anterior pituitary, hypothalamus, striatum, cerebellum, and adrenal. The 14 and 21 day treatments produced a significant 90-110% elevation of CPE mRNA in neurointermediate pituitary. However, the levels of CPE mRNA in the other tissues were not significantly influenced by the haloperidol treatments. This finding indicates that CPE is not co-regulated with peptide hormone mRNAs in all tissues.
KW - Carboxypeptidase E
KW - Carboxypeptidase H
KW - Enkephalin convertase
KW - Neuropeptide biosynthesis
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U2 - 10.1016/0169-328X(93)90162-I
DO - 10.1016/0169-328X(93)90162-I
M3 - Article
C2 - 8361339
AN - SCOPUS:0027253713
SN - 0169-328X
VL - 19
SP - 161
EP - 164
JO - Molecular Brain Research
JF - Molecular Brain Research
IS - 1-2
ER -