DNA sequence-specific recognition by the Saccharomyces cerevisiae 'TATA' binding protein

Promoter-dependent differences in the thermodynamics and kinetics of binding

Victoria Petri, Mark Hsieh, Elizabeth Jamison, Michael D. Brenowitz

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

The equilibrium binding and association kinetics of the Saccharomyces cerevisiae TATA Binding Protein (TBP) to the E4 and Major Late promoters of adenovirus (TATATATA and TATAAAAG, respectively), have been directly compared by quantitative DNase I titration and quench-flow 'footprinting'. The equilibrium binding of TBP to both promoters is described by the equilibrium TBP + DNA('TATA') mutually implies TBP-DNA('TATA'). The salt dependence of TBP binding to both promoters is identical within experimental error while the temperature dependence differs significantly. The observed rate of association follows simple second-order kinetics over the TBP concentration ranges investigated. The salt and temperature dependencies of the second- order association rate constants for TBP binding the two promoters reflect the dependencies determined by equilibrium binding. The TBP-E4 promoter interaction is entropically driven at low temperature and enthalpically driven at high temperature while the TBP-Major Late promoter reaction is entropically driven over virtually the entire temperature range investigated. These data suggest that the reaction mechanisms of TBP-promoter interactions are TATA sequence-specific and provide for differential regulation of promoters as a function of environmental variables.

Original languageEnglish (US)
Pages (from-to)15842-15849
Number of pages8
JournalBiochemistry
Volume37
Issue number45
DOIs
StatePublished - Nov 10 1998

Fingerprint

TATA-Box Binding Protein
DNA sequences
Thermodynamics
Yeast
Saccharomyces cerevisiae
Kinetics
Temperature
Association reactions
Protein Binding
Salts
Saccharomyces cerevisiae Proteins
Deoxyribonuclease I
DNA
Titration
Adenoviridae
Rate constants

ASJC Scopus subject areas

  • Biochemistry

Cite this

DNA sequence-specific recognition by the Saccharomyces cerevisiae 'TATA' binding protein : Promoter-dependent differences in the thermodynamics and kinetics of binding. / Petri, Victoria; Hsieh, Mark; Jamison, Elizabeth; Brenowitz, Michael D.

In: Biochemistry, Vol. 37, No. 45, 10.11.1998, p. 15842-15849.

Research output: Contribution to journalArticle

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