DNA replication in synchronized cultured mammalian cells. V. The temporal order of synthesis of component α DNA during monkey DNA synthesis induced by SV40 virus

Robert J. Parker, Annette M. Tobia, Stephen G. Baum, Carl L. Schildkraut

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Purified simian virus 40 (SV40) was used to induce host DNA replication in contact-inhibited monolayer cultures of African green monkey kidney cells. Approximately 20% of the nuclear DNA of these cells is the simple sequence, component α DNA (Maio, 1971). At the beginning of DNA synthesis induced by SV40 viral infection, the average ratio of the specific radioactivity of component α DNA to that of bulk DNA was 0.18. Similar low specific activity ratios are observed early during the DNA synthetic period following the trypsin release of monkey cells from contact inhibition. These results indicate that when host DNA replication is induced by SV40 viral infection, component α DNA synthesis does not shift to precede the replication of bulk DNA.

Original languageEnglish (US)
Pages (from-to)82-93
Number of pages12
JournalVirology
Volume66
Issue number1
DOIs
StatePublished - 1975

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Simian virus 40
DNA Replication
Haplorhini
Cultured Cells
Viruses
DNA
Virus Diseases
Contact Inhibition
Cercopithecus aethiops
Viral Structures
Trypsin
Radioactivity
Kidney

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

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abstract = "Purified simian virus 40 (SV40) was used to induce host DNA replication in contact-inhibited monolayer cultures of African green monkey kidney cells. Approximately 20{\%} of the nuclear DNA of these cells is the simple sequence, component α DNA (Maio, 1971). At the beginning of DNA synthesis induced by SV40 viral infection, the average ratio of the specific radioactivity of component α DNA to that of bulk DNA was 0.18. Similar low specific activity ratios are observed early during the DNA synthetic period following the trypsin release of monkey cells from contact inhibition. These results indicate that when host DNA replication is induced by SV40 viral infection, component α DNA synthesis does not shift to precede the replication of bulk DNA.",
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AU - Parker, Robert J.

AU - Tobia, Annette M.

AU - Baum, Stephen G.

AU - Schildkraut, Carl L.

PY - 1975

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