DNA repair in congenic mice: Possible influence of a chromosome 4 genetic region on the rate of benzo[a]pyrene-induced DNA adduct removal

M. E T I Boerrigter, Y. Yin, Jan Vijg, J. Y. Wei

Research output: Contribution to journalArticle

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Abstract

An attempt was made to assign mouse lifespan-associated interstrain differences in DNA repair to a specific chromosomal region using a set of congenic mice. The sensitive 32P-postlabeling assay was employed to measure the removal of benzo[a]pyrene-induced DNA adducts in liver DNA of three different chromosome 4 congenic mouse strains: B6.C-H-15(c), B6.C-H-16(c), and B6.C-H-26(c) and the two parental strains, C57B1/6 and BALB/c. The removal of the one main adduct detected, trans-(7R)-N2-[10-(7β,8α,9α- trihydroxy)-7,8,9,10-tetrahydrobenzo(a)-pyrene]-yl-deoxyguanosine (BPDE-N2- dG), in liver DNA of C57B1/6 and BALB/c mice between one and three days after treatment, was approximately 86% and 57%, respectively. The percentage removal of BPDE-N2-dG in two of the three congenic mouse strains, B6.C-H- 16(c) and B6.C-H-26(c), resembled that found in BALB/c, whereas the third strain, B6.C-H-15(c), removed about the same amount as C57B1/6, i.e., approximately 88% of BPDE-N2-dG between one and three days after treatment. The usefulness of congenic mouse strains for identifying genes putatively involved in aging and/or disease susceptibility is discussed.

Original languageEnglish (US)
JournalJournals of Gerontology
Volume48
Issue number1
StatePublished - 1993
Externally publishedYes

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Congenic Mice
Chromosomes, Human, Pair 4
DNA Repair
Deoxyguanosine
Liver
DNA
Disease Susceptibility
benzo(a)pyrene-DNA adduct
Genes
benzo(a)pyrene 7,8-diol-9,10-epoxide-N2-deoxyguanosine

ASJC Scopus subject areas

  • Aging

Cite this

DNA repair in congenic mice : Possible influence of a chromosome 4 genetic region on the rate of benzo[a]pyrene-induced DNA adduct removal. / Boerrigter, M. E T I; Yin, Y.; Vijg, Jan; Wei, J. Y.

In: Journals of Gerontology, Vol. 48, No. 1, 1993.

Research output: Contribution to journalArticle

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