DNA damage by the glycation products of glyceraldehyde 3-phosphate and lysine

E. A. Mullokandov, W. A. Franklin, M. Brownlee

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

In order to evaluate whether base modifications, apurinic/apyrimidinic site formation, strand breaks, or a combination of these lesions results from the interaction of glycation products with DNA, plasmid DNA was first reacted with these products, and then subjected to digestion with endonuclease III and endonuclease IV of Escherichia coli. Analysis of the differential effects of digestions with these enzymes by electrophoresis on agarose gels demonstrated that reactive glycation products produce both base modification and apurinic/apyrimidinic sites in DNA, in addition to the strand breaks observed after incubation with glycation products alone. These types of DNA damage may occur in specific diabetic cells where elevated levels of glycating sugars are associated with pathologic dysfunction.

Original languageEnglish (US)
Pages (from-to)145-149
Number of pages5
JournalDiabetologia
Volume37
Issue number2
StatePublished - Feb 1994

Fingerprint

Glyceraldehyde 3-Phosphate
Lysine
DNA Damage
Digestion
Deoxyribonuclease IV (Phage T4-Induced)
DNA
Agar Gel Electrophoresis
Endonucleases
Plasmids
Escherichia coli
Enzymes

Keywords

  • Amadori products
  • apurinic/apyrimidinic sites
  • base modifications
  • endonucleases
  • strand breaks

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Internal Medicine

Cite this

Mullokandov, E. A., Franklin, W. A., & Brownlee, M. (1994). DNA damage by the glycation products of glyceraldehyde 3-phosphate and lysine. Diabetologia, 37(2), 145-149.

DNA damage by the glycation products of glyceraldehyde 3-phosphate and lysine. / Mullokandov, E. A.; Franklin, W. A.; Brownlee, M.

In: Diabetologia, Vol. 37, No. 2, 02.1994, p. 145-149.

Research output: Contribution to journalArticle

Mullokandov, EA, Franklin, WA & Brownlee, M 1994, 'DNA damage by the glycation products of glyceraldehyde 3-phosphate and lysine', Diabetologia, vol. 37, no. 2, pp. 145-149.
Mullokandov EA, Franklin WA, Brownlee M. DNA damage by the glycation products of glyceraldehyde 3-phosphate and lysine. Diabetologia. 1994 Feb;37(2):145-149.
Mullokandov, E. A. ; Franklin, W. A. ; Brownlee, M. / DNA damage by the glycation products of glyceraldehyde 3-phosphate and lysine. In: Diabetologia. 1994 ; Vol. 37, No. 2. pp. 145-149.
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