DNA damage by the glycation products of glyceraldehyde 3-phosphate and lysine

E. A. Mullokandov, W. A. Franklin, M. Brownlee

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

In order to evaluate whether base modifications, apurinic/apyrimidinic site formation, strand breaks, or a combination of these lesions results from the interaction of glycation products with DNA, plasmid DNA was first reacted with these products, and then subjected to digestion with endonuclease III and endonuclease IV of Escherichia coli. Analysis of the differential effects of digestions with these enzymes by electrophoresis on agarose gels demonstrated that reactive glycation products produce both base modification and apurinic/apyrimidinic sites in DNA, in addition to the strand breaks observed after incubation with glycation products alone. These types of DNA damage may occur in specific diabetic cells where elevated levels of glycating sugars are associated with pathologic dysfunction.

Original languageEnglish (US)
Pages (from-to)145-149
Number of pages5
JournalDiabetologia: Clinical and Experimental Diabetes and Metabolism
Volume37
Issue number2
DOIs
StatePublished - Feb 1994

Keywords

  • Amadori products
  • base modifications
  • endonucleases, apurinic/apyrimidinic sites
  • strand breaks

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

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