Divergent effects of chronic HIV-1 infection on human thymocyte maturation in SCID-hu mice

T. R. Kollmann, A. Kim, M. Pettoello-Mantovani, M. Hachamovitch, A. Rubinstein, M. M. Goldstein, H. Goldstein

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

We have recently developed a modified SCID-hu mouse model in which the implanted human thymus and liver (hu-thy/liv) and human peripheral T cells become infected with HIV-1 after i.p. inoculation. By using this model, we evaluated the effect of HIV-1 infection on thymic maturation and observed that different HIV-1 strains had divergent effects of thymic maturation. Although minimal effects on continued thymopoiesis in the hu-thy/liv implant were observed after chronic infection with two primary patient isolates, HIV- 128 and HIV-159, and with HIV-1(ADA), HIV-1(Ba-L), HIV-1(JR-CSF), HIV- 1(JR-FL), and HIV-1(SF162), significant thymocyte depletion was detected after infection with HIV-1(IIIB) and HIV-1(RF). Thus, the effect of HIV-1 infection on thymocyte maturation may depend upon the strain of HIV-1 infecting the thymus. Despite the minimal effects on thymopoiesis observed in the hu-thy/liv implanted in SCID-hu mice 6 mo after infection with HIV- 128, significant changes were seen in the human T cell population circulating in the peripheral blood of these mice. These changes ranged from an inversion of the CD4/CD8 ratio of peripheral human T cells in some SCID- hu mice to the almost complete depletion of peripheral human T cells observed in other SCID-hu mice. Because these effects were associated with the detection of HIV-1 infection of the peripheral human T cells, these modified SCID-hu mice should prove to be a valuable model for investigating the effects of chronic HIV-1 infection on the peripheral human T cell population.

Original languageEnglish (US)
Pages (from-to)907-921
Number of pages15
JournalJournal of Immunology
Volume154
Issue number2
StatePublished - 1995

Fingerprint

SCID Mice
Thymocytes
HIV Infections
HIV-1
T-Lymphocytes
Thymus Gland
Liver
HIV
CD4-CD8 Ratio
Macrophage Colony-Stimulating Factor
Infection
Population

ASJC Scopus subject areas

  • Immunology

Cite this

Kollmann, T. R., Kim, A., Pettoello-Mantovani, M., Hachamovitch, M., Rubinstein, A., Goldstein, M. M., & Goldstein, H. (1995). Divergent effects of chronic HIV-1 infection on human thymocyte maturation in SCID-hu mice. Journal of Immunology, 154(2), 907-921.

Divergent effects of chronic HIV-1 infection on human thymocyte maturation in SCID-hu mice. / Kollmann, T. R.; Kim, A.; Pettoello-Mantovani, M.; Hachamovitch, M.; Rubinstein, A.; Goldstein, M. M.; Goldstein, H.

In: Journal of Immunology, Vol. 154, No. 2, 1995, p. 907-921.

Research output: Contribution to journalArticle

Kollmann, TR, Kim, A, Pettoello-Mantovani, M, Hachamovitch, M, Rubinstein, A, Goldstein, MM & Goldstein, H 1995, 'Divergent effects of chronic HIV-1 infection on human thymocyte maturation in SCID-hu mice', Journal of Immunology, vol. 154, no. 2, pp. 907-921.
Kollmann TR, Kim A, Pettoello-Mantovani M, Hachamovitch M, Rubinstein A, Goldstein MM et al. Divergent effects of chronic HIV-1 infection on human thymocyte maturation in SCID-hu mice. Journal of Immunology. 1995;154(2):907-921.
Kollmann, T. R. ; Kim, A. ; Pettoello-Mantovani, M. ; Hachamovitch, M. ; Rubinstein, A. ; Goldstein, M. M. ; Goldstein, H. / Divergent effects of chronic HIV-1 infection on human thymocyte maturation in SCID-hu mice. In: Journal of Immunology. 1995 ; Vol. 154, No. 2. pp. 907-921.
@article{c707b5a73fbb400f887bda5ba098091c,
title = "Divergent effects of chronic HIV-1 infection on human thymocyte maturation in SCID-hu mice",
abstract = "We have recently developed a modified SCID-hu mouse model in which the implanted human thymus and liver (hu-thy/liv) and human peripheral T cells become infected with HIV-1 after i.p. inoculation. By using this model, we evaluated the effect of HIV-1 infection on thymic maturation and observed that different HIV-1 strains had divergent effects of thymic maturation. Although minimal effects on continued thymopoiesis in the hu-thy/liv implant were observed after chronic infection with two primary patient isolates, HIV- 128 and HIV-159, and with HIV-1(ADA), HIV-1(Ba-L), HIV-1(JR-CSF), HIV- 1(JR-FL), and HIV-1(SF162), significant thymocyte depletion was detected after infection with HIV-1(IIIB) and HIV-1(RF). Thus, the effect of HIV-1 infection on thymocyte maturation may depend upon the strain of HIV-1 infecting the thymus. Despite the minimal effects on thymopoiesis observed in the hu-thy/liv implanted in SCID-hu mice 6 mo after infection with HIV- 128, significant changes were seen in the human T cell population circulating in the peripheral blood of these mice. These changes ranged from an inversion of the CD4/CD8 ratio of peripheral human T cells in some SCID- hu mice to the almost complete depletion of peripheral human T cells observed in other SCID-hu mice. Because these effects were associated with the detection of HIV-1 infection of the peripheral human T cells, these modified SCID-hu mice should prove to be a valuable model for investigating the effects of chronic HIV-1 infection on the peripheral human T cell population.",
author = "Kollmann, {T. R.} and A. Kim and M. Pettoello-Mantovani and M. Hachamovitch and A. Rubinstein and Goldstein, {M. M.} and H. Goldstein",
year = "1995",
language = "English (US)",
volume = "154",
pages = "907--921",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "2",

}

TY - JOUR

T1 - Divergent effects of chronic HIV-1 infection on human thymocyte maturation in SCID-hu mice

AU - Kollmann, T. R.

AU - Kim, A.

AU - Pettoello-Mantovani, M.

AU - Hachamovitch, M.

AU - Rubinstein, A.

AU - Goldstein, M. M.

AU - Goldstein, H.

PY - 1995

Y1 - 1995

N2 - We have recently developed a modified SCID-hu mouse model in which the implanted human thymus and liver (hu-thy/liv) and human peripheral T cells become infected with HIV-1 after i.p. inoculation. By using this model, we evaluated the effect of HIV-1 infection on thymic maturation and observed that different HIV-1 strains had divergent effects of thymic maturation. Although minimal effects on continued thymopoiesis in the hu-thy/liv implant were observed after chronic infection with two primary patient isolates, HIV- 128 and HIV-159, and with HIV-1(ADA), HIV-1(Ba-L), HIV-1(JR-CSF), HIV- 1(JR-FL), and HIV-1(SF162), significant thymocyte depletion was detected after infection with HIV-1(IIIB) and HIV-1(RF). Thus, the effect of HIV-1 infection on thymocyte maturation may depend upon the strain of HIV-1 infecting the thymus. Despite the minimal effects on thymopoiesis observed in the hu-thy/liv implanted in SCID-hu mice 6 mo after infection with HIV- 128, significant changes were seen in the human T cell population circulating in the peripheral blood of these mice. These changes ranged from an inversion of the CD4/CD8 ratio of peripheral human T cells in some SCID- hu mice to the almost complete depletion of peripheral human T cells observed in other SCID-hu mice. Because these effects were associated with the detection of HIV-1 infection of the peripheral human T cells, these modified SCID-hu mice should prove to be a valuable model for investigating the effects of chronic HIV-1 infection on the peripheral human T cell population.

AB - We have recently developed a modified SCID-hu mouse model in which the implanted human thymus and liver (hu-thy/liv) and human peripheral T cells become infected with HIV-1 after i.p. inoculation. By using this model, we evaluated the effect of HIV-1 infection on thymic maturation and observed that different HIV-1 strains had divergent effects of thymic maturation. Although minimal effects on continued thymopoiesis in the hu-thy/liv implant were observed after chronic infection with two primary patient isolates, HIV- 128 and HIV-159, and with HIV-1(ADA), HIV-1(Ba-L), HIV-1(JR-CSF), HIV- 1(JR-FL), and HIV-1(SF162), significant thymocyte depletion was detected after infection with HIV-1(IIIB) and HIV-1(RF). Thus, the effect of HIV-1 infection on thymocyte maturation may depend upon the strain of HIV-1 infecting the thymus. Despite the minimal effects on thymopoiesis observed in the hu-thy/liv implanted in SCID-hu mice 6 mo after infection with HIV- 128, significant changes were seen in the human T cell population circulating in the peripheral blood of these mice. These changes ranged from an inversion of the CD4/CD8 ratio of peripheral human T cells in some SCID- hu mice to the almost complete depletion of peripheral human T cells observed in other SCID-hu mice. Because these effects were associated with the detection of HIV-1 infection of the peripheral human T cells, these modified SCID-hu mice should prove to be a valuable model for investigating the effects of chronic HIV-1 infection on the peripheral human T cell population.

UR - http://www.scopus.com/inward/record.url?scp=0028869831&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028869831&partnerID=8YFLogxK

M3 - Article

C2 - 7814892

AN - SCOPUS:0028869831

VL - 154

SP - 907

EP - 921

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 2

ER -